2. Academic Validation
  3. cDNA cloning of the mammalian sterol C5-desaturase and the expression in yeast mutant

cDNA cloning of the mammalian sterol C5-desaturase and the expression in yeast mutant

  • Biochim Biophys Acta. 2000 Jan 31;1490(1-2):106-8. doi: 10.1016/s0167-4781(99)00248-1.
S Nishi 1,  H Nishino,  T Ishibashi
Affiliations

Affiliation

  • 1 Department of Biochemistry, Hokkaido University School of Medicine, Sapporo, Japan.
PMID: 10786622 DOI: 10.1016/s0167-4781(99)00248-1
Abstract

Sterol C5-desaturase (SC5D) cDNA is an Enzyme that catalyzes the dehydrogenation to introduce C5-6 double bond into lathosterol in Cholesterol biosynthesis. We have isolated and sequenced the cDNA clones encoding human and mouse SC5D. The functional complementation of a defective yeast mutant proves that the human and mouse cDNA clones encode SC5D. Mammalian SC5D was presumed as an integral membrane protein containing histidine residues conserved also in yeasts and plant.

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