2. Academic Validation
  3. Isolation and characterization of a human thiamine pyrophosphokinase cDNA

Isolation and characterization of a human thiamine pyrophosphokinase cDNA

  • Biochim Biophys Acta. 2001 Jan 26;1517(2):293-7. doi: 10.1016/s0167-4781(00)00247-5.
K Nosaka 1,  M Onozuka,  N Kakazu,  S Hibi,  H Nishimura,  H Nishino,  T Abe
Affiliations

Affiliation

  • 1 Department of Chemistry, Kyoto Prefectural University of Medicine, Kita-ku, Kyoto 603-8334, Japan. knosaka@basic.kpu-m.ac.jp
PMID: 11342111 DOI: 10.1016/s0167-4781(00)00247-5
Abstract

A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked Enzyme activity was detected in the Bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.

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