1. Academic Validation
  2. Production of bioactive triterpenes by Eriobotrya japonica calli

Production of bioactive triterpenes by Eriobotrya japonica calli

  • Phytochemistry. 2002 Feb;59(3):315-23. doi: 10.1016/s0031-9422(01)00455-1.
Shoko Taniguchi 1 Yoko Imayoshi Eri Kobayashi Yoshie Takamatsu Hideyuki Ito Tsutomu Hatano Hiroshi Sakagami Harukuni Tokuda Hoyoku Nishino Daigo Sugita Susumu Shimura Takashi Yoshida
Affiliations

Affiliation

  • 1 Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Abstract

Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced Triterpenes in large amounts (CA. 50 mg/g dry wt). Nine Triterpenes were characterized as ursolic acid, oleanolic acid, 2alpha-hydoxyursolic acid, maslinic acid, tormentic acid, 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the Triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2alpha, 19alpha-Dihydroxy-3-oxo-urs-12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.

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