2. Academic Validation
  3. Doxorubicin inhibits human DNA topoisomerase I

Doxorubicin inhibits human DNA topoisomerase I

  • Cancer Chemother Pharmacol. 1992;30(2):123-5. doi: 10.1007/BF00686403.
P D Foglesong 1,  C Reckord,  S Swink
Affiliations

Affiliation

  • 1 Biology Department, Rutgers University, Camden, New Jersey 08102.
PMID: 1318169 DOI: 10.1007/BF00686403
Abstract

Purified human DNA Topoisomerase I was assayed quantitatively by Enzyme titrations with supercoiled pHC624 DNA in the presence of 0-2.0 microM doxorubicin. Supercoiled and relaxed DNAs were resolved by Agarose gel electrophoresis in the presence of ethidium bromide, and the percentage of conversion of supercoiled DNA to relaxed DNA was quantified by scanning microdensitometry. The inhibition of DNA Topoisomerase I activity was measured at varying concentrations of doxorubicin. Doxorubicin inhibited Enzyme activity at an IC50 value (the concentration required to inhibit 50% of the total activity) of 0.8 microM. Similar inhibition was observed for daunomycin, a structurally related anthracycline antitumor drug. These results indicate that anthracyclines inhibit human DNA Topoisomerase I activity at concentrations that cause DNA damage and cytotoxicity in vivo.

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