2. Academic Validation
  3. C/EBPdelta and C/EBPgamma bind the CCAAT-box in the human beta-globin promoter and modulate the activity of the CACC-box binding protein, EKLF

C/EBPdelta and C/EBPgamma bind the CCAAT-box in the human beta-globin promoter and modulate the activity of the CACC-box binding protein, EKLF

  • Biochim Biophys Acta. 2005 May 25;1729(1):74-80. doi: 10.1016/j.bbaexp.2005.03.004.
Christopher T Gordon 1,  Vanessa J Fox,  Suzana Najdovska,  Andrew C Perkins
Affiliations

Affiliation

  • 1 Institute for Molecular Bioscience, University of Queensland, St. Lucia, 4072, QLD, Australia.
PMID: 15833715 DOI: 10.1016/j.bbaexp.2005.03.004
Abstract

Developmental- and tissue-specific expression of globin genes is mediated by a few key elements within the proximal promoter of each gene. DNA-binding assays previously identified NF-Y, GATA-1, C/EBPbeta and C/EBPgamma as candidate regulators of beta-globin transcription via the CCAAT-box, a promoter element situated between CACC- and TATA-boxes. We have identified C/EBPdelta as an additional beta-globin CCAAT-box binding protein. In reporter assays, we show that C/EBPdelta can co-operate with EKLF, a CACC-box binding protein, to activate the beta-globin promoter, whereas C/EBPgamma inhibits the transcriptional activity of EKLF in this assay.

Figures