2. Academic Validation
  3. Delphinidin 3-sambubioside, a Hibiscus anthocyanin, induces apoptosis in human leukemia cells through reactive oxygen species-mediated mitochondrial pathway

Delphinidin 3-sambubioside, a Hibiscus anthocyanin, induces apoptosis in human leukemia cells through reactive oxygen species-mediated mitochondrial pathway

  • Arch Biochem Biophys. 2005 Aug 1;440(1):101-9. doi: 10.1016/j.abb.2005.06.002.
De-Xing Hou 1,  Xuhui Tong,  Norihiko Terahara,  Dong Luo,  Makoto Fujii
Affiliations

Affiliation

  • 1 Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan. hou@chem.agri.kagoshima-u.ac.jp
PMID: 16018963 DOI: 10.1016/j.abb.2005.06.002
Abstract

Delphinidin 3-sambubioside (Dp3-Sam), a Hibiscus anthocyanin, was isolated from the dried calices of Hibiscus sabdariffa L. Dp3-Sam could induce a dose-dependent Apoptosis in human leukemia cells (HL-60) as characterized by cell morphology, DNA fragmentation, activation of Caspase-3, -8, and -9, and inactivation of poly(ADP)ribose polymerase (PARP). Molecular data showed that Dp3-Sam induced Bid truncation, mitochondrial membrane potential (DeltaPsi(m)) loss, and cytochrome c release from mitochondria to cytosol. Moreover, Dp3-Sam caused a time- and dose-dependent elevation of intracellular Reactive Oxygen Species (ROS) level in HL-60 cells, and Antioxidants such as N-acetyl-L-cysteine (NAC) and catalase could effectively block Dp3-Sam-induced ROS generation, Caspase-3 activity, and DNA fragmentation. These data indicate that Dp3-Sam might induce Apoptosis in HL-60 cells through a ROS-mediated mitochondrial dysfunction pathway. These findings enhance our understanding for Anticancer function of Hibiscus anthocyanins in herbal medicine.

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