Latrunculin a has a strong anticancer effect in a peritoneal dissemination model of human gastric cancer in mice

Peritoneal dissemination of gastric Cancer is a refractory disease. This paper focuses on the efficacy of actin-binding marine Macrolide latrunculin A, which quickly inhibits actin polymerization and disrupts the function of the actin Cytoskeleton. The effects of latrunculin A on cell viability in vitro were evaluated by treatment of MKN45 or NUGC-4 cell cultures. An in vitro viability assay demonstrated an Anticancer effect of latrunculin A in a dose-dependent manner. Latrunculin A induced acute cell injury and programmed cell death through activating the Caspase-3/7 pathway. In vivo, MKN45 or NUGC-4 cells were intraperitoneally inoculated into nude mice, as a model of peritoneal dissemination. Intraperitoneal (i.p.) injection of latrunculin A significantly improved survival rate in mice without any major side-effects. Data indicated that latrunculin A has strong Anticancer effects, and it may be a new candidate i.p. drug against peritoneal dissemination of gastric Cancer in humans.