2. Academic Validation
  3. Validation of HPLC-MS/MS methods for analysis of loxapine, amoxapine, 7-OH-loxapine, 8-OH-loxapine and loxapine N-oxide in human plasma

Validation of HPLC-MS/MS methods for analysis of loxapine, amoxapine, 7-OH-loxapine, 8-OH-loxapine and loxapine N-oxide in human plasma

  • Bioanalysis. 2010 Dec;2(12):1989-2000. doi: 10.4155/bio.10.156.
Jennifer S D Zimmer 1,  Shane R Needham,  Chad D Christianson,  Crystal M Piekarski,  Chrystal N Sheaff,  Keith Huie,  Andrea R Reed,  Lori Takahashi
Affiliations

Affiliation

  • 1 Alturas Analytics, Inc., 1324 Alturas Drive, Moscow, ID 83843, USA. jzimmer@alturasanalytics.com
PMID: 21110742 DOI: 10.4155/bio.10.156
Abstract

Background: Two ESI-LC-MS/MS methods were validated for the quantitative analysis of loxapine, amoxapine, 7-OH-loxapine, 8-OH-loxapine and loxapine N-oxide in human K(2)EDTA plasma. Cation-exchange solid-phase extraction (SPE) was used to extract loxapine, amoxapine and the two hydroxylated metabolites, and organic precipitation was used to quantify loxapine N-oxide.

Results: Both methods were shown to be accurate (±13%), intra-assay precision was less than 15%, and inter-assay precision was less than 10% in all instances across the entire dynamic range of the assays (0.0500-50.0 ng/ml for the SPE method and 0.100-25.0 ng/ml for the precipitation method).

Conclusion: The validated methods for loxapine, amoxapine, 7-OH-loxapine, 8-OH-loxapine and loxapine N-oxide have been used to successfully support clinical trials.

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