A novel binding protein of single immunoglobulin IL-1 receptor-related molecule: Paralemmin-3

Biochem Biophys Res Commun. 2011 Jan 28;404(4):1029-33. doi: 10.1016/j.bbrc.2010.12.104.

Xuxin Chen ¹, Xueling Wu, Yunfeng Zhao, Guansong Wang, Jian Feng, Qi Li, Guisheng Qian

Affiliations

Affiliation

1 Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China.

PMID: 21187075 DOI: 10.1016/j.bbrc.2010.12.104

Abstract

Previous studies have shown that single immunoglobulin IL-1 receptor-related molecule (SIGIRR) is a negative regulator of Toll-Interleukin-1 receptor signaling. Nevertheless, the molecular mechanism of the negatively regulatory effect of SIGIRR remains unknown. Using a yeast two-hybrid screen, we identified paralemmin-3 (PALM3) as a novel binding protein of SIGIRR. This interaction of SIGIRR with PALM3 was confirmed by coimmunoprecipitation in mammalian cells. In addition, the PALM3 mRNA expression was upregulated by lipopolysaccharide (LPS)-stimulation in a human alveolar epithelial cell line (A549 cells). Furthermore, silencing PALM3 by RNA interference inhibited the release of inflammatory cytokines in A549 cells after LPS-stimulation. These results suggest that PALM3 may function as an adaptor in the LPS- Toll-like Receptor 4 signaling and the interaction of SIGIRR with PALM3 may partly account for the mechanism of the negatively regulatory effect of SIGIRR.

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