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  2. Separation and purification of sulforaphene from radish seeds using macroporous resin and preparative high-performance liquid chromatography

Separation and purification of sulforaphene from radish seeds using macroporous resin and preparative high-performance liquid chromatography

  • Food Chem. 2013 Jan 15;136(2):342-7. doi: 10.1016/j.foodchem.2012.08.082.
Pengqun Kuang 1 Dan Song Qipeng Yuan Rui Yi Xinhua Lv Hao Liang
Affiliations

Affiliation

  • 1 State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Abstract

This present study described a rapid and cost-effective method for the separation and purification of natural sulforaphene from radish seeds by SP-700 macroporous resin and preparative high-performance liquid chromatography (HPLC). Sulforaphene with high purity and recovery was obtained by preparative HPLC with a C18 column and 30% methanol in ultra-pure water as the mobile phase. 12.5 kg of radish seeds, which contained 87.5 g of sulforaphene, produced 117.5 g of sulforaphene-rich extract of 65.8% sulforaphene after primary separation by SP-700 macroporous resin. 5.9 g of 96.5% sulforaphene was obtained from 9.5 g of the sulforaphene-rich extract after purification by preparative HPLC. The purified compound was assessed by analytical HPLC and characterised by ESI/MS, (1)H NMR and (13)C NMR. Standard curve was developed using the purified sulforaphene to allow quantification of sulforaphene in the extracts of radish seeds by analytical HPLC.

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