2. Apoptosis Protein Tyrosine Kinase/RTK JAK/STAT Signaling MAPK/ERK Pathway Stem Cell/Wnt NF-κB Cell Cycle/DNA Damage Cytoskeleton
  3. Apoptosis EGFR ERK NF-κB Microtubule/Tubulin
  4. Sulforaphene

Sulforaphene, isolated from radish seeds, exhibits an ED50 against velvetleaf seedlings approximately 2 x 10-4 M. Sulforaphene promotes cancer cells apoptosis and inhibits migration via inhibiting EGFR, p-ERK1/2, NF‐κB and other signals.

For research use only. We do not sell to patients.

Sulforaphene Chemical Structure

Sulforaphene Chemical Structure

CAS No. : 592-95-0

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25 mg USD 220 In-stock
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Based on 1 publication(s) in Google Scholar

Sulforaphene Related Antibodies

Top Publications Citing Use of Products

View All EGFR Isoform Specific Products:

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

View All ERK Isoform Specific Products:

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ERK ERK1 ERK2 ERK5 ERK8

View All NF-κB Isoform Specific Products:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

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Description

Sulforaphene, isolated from radish seeds, exhibits an ED50 against velvetleaf seedlings approximately 2 x 10-4 M. Sulforaphene promotes cancer cells apoptosis and inhibits migration via inhibiting EGFR, p-ERK1/2, NF‐κB and other signals[1][2][3][4].

Cellular Effect
Cell Line Type Value Description References
A-375 IC50
18.74 μM
Compound: SFE
Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay
[PMID: 30282324]
A549 IC50
9.33 μM
Compound: SFE
Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay
[PMID: 30282324]
HCT-116 IC50
31.92 μM
Compound: SFE
Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay
[PMID: 30282324]
HeLa IC50
85.05 μM
Compound: SFE
Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay
Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay
[PMID: 30282324]
SMMC-7721 IC50
6.41 μM
Compound: SFE
Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay
Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay
[PMID: 30282324]
In Vitro

Sulforaphene (0-80 μM, 48 h) inhibits HCC (HepG2 and Hep3B cells) cell viability and induces apoptosis[4].
Sulforaphene (0-40 μM) sensitizes radiosensitivity of HepG2 and Hep3B cells, and promotes radiation-induced cell death[4].
Sulforaphene (0-10 μM, 24 h) inhibits SUM159 cell migration and invasion by inhibiting Hedgehog signaling[5].
Sulforaphene (5 μM, 24 h) inhibits microtubule polymerization in HCT116 and HT-29 cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sulforaphene Related Antibodies

Western Blot Analysis[5]

Cell Line: HepG2 and Hep3B cell
Concentration: 0-40 μM
Incubation Time: 48 h
Result: Increased Bax expression and reduced Bcl-2 expression.
Inhibited expression of NF-𝜅B-dependent genes (COX-2, iNos, and cyclinD1).
In Vivo

Sulforaphene (1 and 5 mg/kg, i.p., everyday) inhibits tumor growth in a mouse HCT116 xenograft model[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mouse HCT116 xenograft model[6]
Dosage: 1 and 5 mg/kg
Administration: i.p., everyday
Result: Inhibited tumor growth, and increased CDK1, MK2, and p38 phosphorylation.
Molecular Weight

175.27

Formula

C6H9NOS2
CAS No.
Appearance

Liquid (Density: 1.16±0.1 g/cm3)

Color

Colorless to light yellow

SMILES

O=S(/C=C/CCN=C=S)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (570.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7055 mL 28.5274 mL 57.0548 mL
5 mM 1.1411 mL 5.7055 mL 11.4110 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.80%

SDS (393 KB)

COA (243 KB) HNMR (252 KB) LCMS (255 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.7055 mL 28.5274 mL 57.0548 mL 142.6371 mL
5 mM 1.1411 mL 5.7055 mL 11.4110 mL 28.5274 mL
10 mM 0.5705 mL 2.8527 mL 5.7055 mL 14.2637 mL
15 mM 0.3804 mL 1.9018 mL 3.8037 mL 9.5091 mL
20 mM 0.2853 mL 1.4264 mL 2.8527 mL 7.1319 mL
25 mM 0.2282 mL 1.1411 mL 2.2822 mL 5.7055 mL
30 mM 0.1902 mL 0.9509 mL 1.9018 mL 4.7546 mL
40 mM 0.1426 mL 0.7132 mL 1.4264 mL 3.5659 mL
50 mM 0.1141 mL 0.5705 mL 1.1411 mL 2.8527 mL
60 mM 0.0951 mL 0.4755 mL 0.9509 mL 2.3773 mL
80 mM 0.0713 mL 0.3566 mL 0.7132 mL 1.7830 mL
100 mM 0.0571 mL 0.2853 mL 0.5705 mL 1.4264 mL
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Sulforaphene Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulforaphene592-95-0ApoptosisEGFRERKNF-κBMicrotubule/TubulinEpidermal growth factor receptorErbB-1HER1Extracellular signal regulated kinasesNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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