1. Academic Validation
  2. Cardenolide glycosides from Elaeodendron australe var. integrifolium

Cardenolide glycosides from Elaeodendron australe var. integrifolium

  • Phytochemistry. 2014 Feb:98:160-3. doi: 10.1016/j.phytochem.2013.11.024.
Mark S Butler 1 Leanne Towerzey 1 Ngoc B Pham 1 Edward Hyde 1 Sao Khemar Wadi 1 Gordon P Guymer 2 Ronald J Quinn 3
Affiliations

Affiliations

  • 1 Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia.
  • 2 Queensland Herbarium, Department of Science, Information Technology, Innovation and the Arts, Brisbane Botanic Gardens, Brisbane, Australia.
  • 3 Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia. Electronic address: r.quinn@griffith.edu.au.
Abstract

Extracts from dried leaf and stems of Elaeodendron australe var. integrifolium (Celastraceae) collected in South East Queensland, Australia, were active in an assay that measured CA(2+) driven expression of IL-2/luciferase designed to identify inhibitors of the ICRAC channel. Bioassay-guided isolation using C18 and polyamide column chromatography, HPLC (Phenyl and C18) and centrifugal partition chromatography (CPC) led to the isolation of digitoxigenin (1) and three cardenolide glycosides, glucoside 2, quinovoside 3 and the new natural product xyloside 4, as the active components with low nM activity in the reporter assay.

Keywords

Cardenolide; Celastraceae; Centrifugal partition chromatography; Elaeodendron australe var. integrifolium; Interleukin-2 (IL-2)/luciferase inhibitor; Ion channel; Na(+)/K(+)-ATPase.

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