2. Academic Validation
  3. Design, synthesis, biological evaluation, and comparative docking study of 1,2,4-triazolones as CB1 receptor selective antagonists

Design, synthesis, biological evaluation, and comparative docking study of 1,2,4-triazolones as CB1 receptor selective antagonists

  • Eur J Med Chem. 2014 Mar 3:74:73-84. doi: 10.1016/j.ejmech.2013.12.018.
Shuang Han 1,  Fei-Fei Zhang 2,  Xin Xie 2,  Jian-Zhong Chen 3
Affiliations

Affiliations

  • 1 College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
  • 2 CAS Key Laboratory of Receptor Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
  • 3 College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: chjz@zju.edu.cn.
PMID: 24445310 DOI: 10.1016/j.ejmech.2013.12.018
Abstract

Cannabinoids are potentially useful for the treatment of several diseases. In the present work, we report the syntheses and biological evaluations of 1,2,4-triazolone derivatives designed using a combined approach of scaffold hopping and pharmacophore-oriented method. These compounds exhibited interesting antagonistic activity to the cannabinoid CB1 receptor. The preliminary structure-activity relationships were further discussed. In addition, docking simulations were performed on the good bioactive compound 5c and the low potent compound 5d, respectively, on the basis of homology models of the CB1 and CB2 receptors, which were constructed based on human β2-adrenoreceptor and optimized in a membrane environment by MD simulations. Calculation of the binding modes gave us insights into the structural requirements for improving the Cannabinoid Receptor bioactivity and selectivity.

Keywords

1,2,4-Triazolone; Antagonist; Cannabinoid receptors; Homology model; Scaffold hopping.

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