2. Academic Validation
  3. Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots

Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots

  • J Ginseng Res. 2014 Apr;38(2):116-22. doi: 10.1016/j.jgr.2013.10.002.
Jin-Gyeong Cho 1,  Seo-Ji In 1,  Ye-Jin Jung 1,  Byeong-Ju Cha 1,  Dae-Young Lee 2,  Yong-Bum Kim 3,  Myeonghun Yeom 4,  Nam-In Baek 1
Affiliations

Affiliations

  • 1 Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
  • 2 Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Korea.
  • 3 Technology Services Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, Korea.
  • 4 Amorepacific Corporation Research and Development Center, Yongin, Korea.
PMID: 24748835 DOI: 10.1016/j.jgr.2013.10.002
Abstract

Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as (1)H-(1)H correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published (1)H- and (13)C-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.

Keywords

HPLC; NMR; Panax ginseng; fast atom bombardment/MS; triol ginsenoside.

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