2. Academic Validation
  3. Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets

Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets

  • RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114.
Thalia A Farazi 1,  Carl S Leonhardt 1,  Neelanjan Mukherjee 2,  Aleksandra Mihailovic 1,  Song Li 3,  Klaas E A Max 1,  Cindy Meyer 1,  Masashi Yamaji 1,  Pavol Cekan 1,  Nicholas C Jacobs 2,  Stefanie Gerstberger 1,  Claudia Bognanni 1,  Erik Larsson 4,  Uwe Ohler 2,  Thomas Tuschl 1
Affiliations

Affiliations

  • 1 Laboratory of RNA Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10065, USA.
  • 2 Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
  • 3 Biology Department, Duke University, Durham, North Carolina 27708, USA.
  • 4 Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, SE-405 30, Sweden.
PMID: 24860013 DOI: 10.1261/rna.045005.114
Abstract

Recent studies implicated the RNA-binding protein with multiple splicing (RBPMS) family of proteins in oocyte, retinal ganglion cell, heart, and gastrointestinal smooth muscle development. These RNA-binding proteins contain a single RNA recognition motif (RRM), and their targets and molecular function have not yet been identified. We defined transcriptome-wide RNA targets using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in HEK293 cells, revealing exonic mature and intronic pre-mRNA binding sites, in agreement with the nuclear and cytoplasmic localization of the proteins. Computational and biochemical approaches defined the RNA recognition element (RRE) as a tandem CAC trinucleotide motif separated by a variable spacer region. Similar to other mRNA-binding proteins, RBPMS family of proteins relocalized to cytoplasmic stress granules under oxidative stress conditions suggestive of a support function for mRNA localization in large and/or multinucleated cells where it is preferentially expressed.

Keywords

PAR-CLIP; RBPMS; RNA recognition element; RNA-binding protein.

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