2. Academic Validation
  3. MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung's disease

MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung's disease

  • BMC Gastroenterol. 2014 Jul 9;14:123. doi: 10.1186/1471-230X-14-123.
Hao Lei,  Junwei Tang,  Hongxing Li,  Hongwei Zhang,  Changgui Lu,  Huan Chen,  Wei Li,  Yankai Xia,  Weibing Tang 1
Affiliations

Affiliation

  • 1 State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China. twbcn@163.com.
PMID: 25007945 DOI: 10.1186/1471-230X-14-123
Abstract

Background: Hirschsprung's disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR.

Methods: In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR and 66 controls without HSCR. Transwell, Cell Counting Kit-8 (CCK-8) and flow cytometry assay were employed to detect the function role of miR-195 in vitro.

Results: Our results showed that expression levels of miR-195 from patients with HSCR were significantly higher than control group; along with aberrant lower expression levels of digestive-organ expansion factor (DIEXF) were tested. Increased level of miR-195 could suppress the level of DIEXF in cell, which induced the impairment of cell migration and proliferation.

Conclusions: Aberrant expression of miR-195 may involved in the pathogenesis of HSCR by down-regulated the level of DIEXF.

Figures