2. Academic Validation
  3. Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice

Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice

  • J Nutr Biochem. 2015 Jan;26(1):57-63. doi: 10.1016/j.jnutbio.2014.09.004.
Kazuaki Yoshinaga 1,  Keiichi Sasaki 2,  Hiroyuki Watanabe 3,  Koji Nagao 4,  Nao Inoue 4,  Bungo Shirouchi 4,  Teruyoshi Yanagita 5,  Toshiharu Nagai 6,  Hoyo Mizobe 6,  Koichi Kojima 6,  Fumiaki Beppu 2,  Naohiro Gotoh 7
Affiliations

Affiliations

  • 1 Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan; Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
  • 2 Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
  • 3 Department of Health Science, University of Kochi, 2751-1 Ike, Kochi-shi, Kochi 781-0111, Japan.
  • 4 Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga-shi, Saga 840-8502, Japan.
  • 5 Department of Health and Nutrition Science, Nishikyushu University, 4490-9 Ozaki, Kanzaki-machi, Kanzaki-shi, Saga 842-8585, Japan.
  • 6 Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
  • 7 Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: ngotoh@kaiyodai.ac.jp.
PMID: 25448607 DOI: 10.1016/j.jnutbio.2014.09.004
Abstract

The present study investigated the effects of binding position of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to triacylglycerol (TAG) on lipid metabolism in C57BL/6J mice. Mice were treated with pure TAG positional isomers, including 1,2(2,3)-dipalmitoyl-3(1)-eicosapentaenoyl glycerol, 1,3-dipalmitoyl-2-eicosapentaenoyl glycerol, 1,2(2,3)-dipalmitoyl-3(1)-docosahexaenoyl glycerol, and 1,3-dipalmitoyl-2-docosahexaenoyl glycerol. Compared to DHA bound to the α-position of TAG, DHA bound to the β-position more effectively inhibited fatty acid synthetic Enzymes and cholesterol-metabolism Enzymes and thus reduced TAG and Cholesterol concentrations in the serum and liver. EPA bound to the α-position of TAG, but not EPA bound to the β-position of TAG, significantly decreased hepatic Cholesterol concentrations. Additionally, EPA bound to the α-position of TAG increased the ratio of PGI2 to TXA2 to a higher degree than EPA bound to the β-position. These results suggested that the binding position of EPA and DHA to TAG affected TAG and Cholesterol metabolism as well as eicosanoid production in C57BL/6J mice.

Keywords

C57BL/6J mouse; Docosahexaenoic acid; Eicosapentaenoic acid; Lipid metabolism; Positional isomer; Triacylglycerol.

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