2. Academic Validation
  3. Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages

Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages

  • Bioorg Med Chem Lett. 2015 Feb 15;25(4):960-2. doi: 10.1016/j.bmcl.2014.12.012.
Jin Woo Lee 1,  Ji Hoon Lee 1,  Chul Lee 2,  Qinghao Jin 1,  Dongho Lee 3,  Youngsoo Kim 1,  Jin Tae Hong 1,  Mi Kyeong Lee 1,  Bang Yeon Hwang 4
Affiliations

Affiliations

  • 1 College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
  • 2 College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea; Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk 580-185, Republic of Korea.
  • 3 College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
  • 4 College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea. Electronic address: byhwang@chungbuk.ac.kr.
PMID: 25592708 DOI: 10.1016/j.bmcl.2014.12.012
Abstract

Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4',7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0μM in RAW 264.7 macrophages.

Keywords

Leguminosae; Nitric oxide production inhibitor; Pterocarpan; Sophora tonkinensis.

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