2. Academic Validation
  3. HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma

HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma

  • Biochem Biophys Res Commun. 2015 Mar 20;458(4):849-55. doi: 10.1016/j.bbrc.2015.02.042.
Kun Gao 1,  Chen Xu 1,  Xiaofeng Jin 1,  Reziya Wumaier 1,  Jian Ma 2,  Jingtao Peng 2,  Yuqi Wang 1,  Yan Tang 1,  Long Yu 3,  Pingzhao Zhang 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
  • 2 Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, PR China.
  • 3 State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China. Electronic address: longyu@fudan.edu.cn.
  • 4 State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China; Shanghai Cancer Center, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, PR China. Electronic address: pzzhang@fudan.edu.cn.
PMID: 25689719 DOI: 10.1016/j.bbrc.2015.02.042
Abstract

HDGFRP2 (HRP-2) belongs to the Hepatoma-derived growth factor (HDGF)-related proteins (HRPs) family, which are characterized by a conserved HATH/PWWP domain at a well-conserved region of the N-terminus. However, the cellular function of HRP-2 remains unknown. In this study, we showed for the first time that HRP-2 is frequently overexpressed in human HCC tissues at mRNA and protein levels. We further showed that HRP-2 can promote HCC cells growth in vitro and xenograft tumors in vivo. Using protein affinity purification methods, we searched for functional partners of HRP-2, and found that HRP-2 interacts with various proteins known to be involved in transcription elongation and processing. Furthermore, we demonstrate HRP-2 interacts and co-localizes with RNA processing regulator IWS1, and positively regulated the mRNA level of Cyclin D1. Together, our study suggests HRP-2 may act as an mRNA processing co-factor to promote cells growth by regulating the mRNA of key oncogenes, which can be explored further for Cancer treatment.

Keywords

Cells growth; Hepatocellular carcinoma; Protein interaction; mRNA processing.

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