Effect of recombinant human endostatin on the expression of c-Myc and bFGF in mouse gastric cancer cells

The aim of this study was to observe the effects of re-combinant human endostatin on the proliferation and Apoptosis of mouse gastric Cancer cells, and explore some possible mechanisms of recom-binant human endostatin inhibition of Cancer. A murine gastric Cancer xenograft model was established. A total of 20 mice were divided into two groups (control and experimental groups). The expression of c-Myc and basic Fibroblast Growth Factor (bFGF) was determined by reverse transcription-polymerase chain reaction, Western blotting, and immu-nohistochemical staining methods. Tumor volume was measured and a growth curve was calculated. The tumor diameter in the experimental group was significantly smaller than that in the control group after treat-ment with endostatin for 21 days. The expression levels of c-Myc and bFGF in the experimental group were significantly lower than those of the control group (P < 0.05). There was a positive correlation between the expression of c-Myc and bFGF in the experimental group. Microvessel density was significantly inhibited in the experimental group (P < 0.05). These results demonstrated that recombinant human endostatin could in-hibit tumor metastasis by inhibition of the expression of c-Myc and bFGF in gastric Cancer tissue as well as by inhibition of angiogenesis.