2. Academic Validation
  3. Neuroprotective effects of Kukoamine B against hydrogen peroxide-induced apoptosis and potential mechanisms in SH-SY5Y cells

Neuroprotective effects of Kukoamine B against hydrogen peroxide-induced apoptosis and potential mechanisms in SH-SY5Y cells

  • Environ Toxicol Pharmacol. 2015 Jul;40(1):230-40. doi: 10.1016/j.etap.2015.06.017.
Xiao-Long Hu 1,  Yi-Xuan Niu 2,  Qiao Zhang 2,  Xing Tian 2,  Ling-Yue Gao 2,  Li-Ping Guo 2,  Wei-Hong Meng 3,  Qing-Chun Zhao 4
Affiliations

Affiliations

  • 1 Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang 110840, China; Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China.
  • 2 Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China.
  • 3 Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang 110840, China.
  • 4 Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang 110840, China. Electronic address: zhaoqingchun1967@163.com.
PMID: 26164594 DOI: 10.1016/j.etap.2015.06.017
Abstract

Oxidative stress mediates the cell damage in several neurodegenerative diseases, including multiple sclerosis, Alzheimer's disease (AD) and Parkinson's disease (PD). This study aimed at investigating the protective effects of Kukoamine B (KuB) against hydrogen peroxide (H2O2) induced cell injury and potential mechanisms in SH-SY5Y cells. Our results revealed that treatment with KuB prior to H2O2 exposure effectively increased the cell viability, and restored the mitochondria membrane potential (MMP). Furthermore, KuB enhanced the antioxidant Enzyme activities of superoxide dismutase (SOD), catalase (CAT), and Glutathione Peroxidase (GSH-Px) and decreased the malondialdehyde (MDA) content. Moreover, KuB minimized the ROS formation and inhibited mitochondria-apoptotic pathway, MAPKs (p-p38, p-JNK, p-ERK) pathways, but activated PI3K-AKT pathway. In conclusion, we believed that KuB may potentially serve as an agent for prevention of several human neurodegenerative and Other disorders caused by oxidative stress.

Keywords

Hydrogen peroxide; Kukoamine B; Neuroprotection; Oxidative stress; SH-SY5Y cells.

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