2. Academic Validation
  3. Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway

Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway

  • Evid Based Complement Alternat Med. 2015;2015:298635. doi: 10.1155/2015/298635.
Chian-Jiun Liou 1,  Xuan-Yu Lai 2,  Ya-Ling Chen 3,  Chia-Ling Wang 4,  Ciao-Han Wei 4,  Wen-Chung Huang 5
Affiliations

Affiliations

  • 1 Department of Nursing, Chang Gung University of Science and Technology, 261 Wen-Hwa 1st Road, Kwei-Shan, Taoyuan, Taiwan.
  • 2 Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, 261 Wen-Hwa 1st Road, Kwei-Shan, Taoyuan, Taiwan ; Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Kwei-Shan, Taoyuan, Taiwan.
  • 3 Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, 261 Wen-Hwa 1st Road, Kwei-Shan, Taoyuan, Taiwan.
  • 4 Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Kwei-Shan, Taoyuan, Taiwan.
  • 5 Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Kwei-Shan, Taoyuan, Taiwan ; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kwei-Shan, Taoyuan, Taiwan.
PMID: 26413119 DOI: 10.1155/2015/298635
Abstract

Ginkgolide C, isolated from Ginkgo biloba leaves, is a diterpene lactone derivative [corrected] reported to have multiple biological functions, from decreased platelet aggregation to ameliorating Alzheimer disease. The study aim was to evaluate the antiadipogenic effect of ginkgolide C in 3T3-L1 adipocytes. Ginkgolide C was used to treat differentiated 3T3-L1 cells. Cell supernatant was collected to assay glycerol release, and cells were lysed to measure protein and gene expression related to adipogenesis and lipolysis by western blot and Real-Time PCR, respectively. Ginkgolide C significantly suppressed lipid accumulation in differentiated adipocytes. It also decreased adipogenesis-related transcription factor expression, including Peroxisome Proliferator-activated Receptor and CCAAT/enhancer-binding protein. Furthermore, ginkgolide C enhanced adipose triglyceride Lipase and hormone-sensitive Lipase production for lipolysis and increased phosphorylation of AMP-activated protein kinase (AMPK), resulting in decreased activity of Acetyl-CoA Carboxylase for fatty acid synthesis. In coculture with an AMPK Inhibitor (compound C), ginkgolide C also improved activation of Sirtuin 1 and phosphorylation of AMPK in differentiated 3T3-L1 cells. The results suggest that ginkgolide C is an effective flavone for increasing lipolysis and inhibiting adipogenesis in adipocytes through the activated AMPK pathway.

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