2. Academic Validation
  3. Forsythiaside A Exhibits Anti-inflammatory Effects in LPS-Stimulated BV2 Microglia Cells Through Activation of Nrf2/HO-1 Signaling Pathway

Forsythiaside A Exhibits Anti-inflammatory Effects in LPS-Stimulated BV2 Microglia Cells Through Activation of Nrf2/HO-1 Signaling Pathway

  • Neurochem Res. 2016 Apr;41(4):659-65. doi: 10.1007/s11064-015-1731-x.
Yue Wang 1 2,  Hongfei Zhao 3,  Chuangxin Lin 4,  Jie Ren 5,  Shizhong Zhang 6
Affiliations

Affiliations

  • 1 The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.
  • 2 Department of Neurosurgery, No. 208 Hospital of PLA, Changchun, China.
  • 3 Department of Encephalopathy, The Affiliated Hospital of Changchun Chinese Medicine University, Changchun, China.
  • 4 The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • 5 Nursing Department, No. 461 Hospital of PLA, Changchun, China.
  • 6 The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China. zhangshizhongwb36@163.com.
PMID: 26498935 DOI: 10.1007/s11064-015-1731-x
Abstract

Inflammation and oxidative stress have been reported to play critical roles in the pathogenesis of neurodegenerative disease. Forsythiaside A, a phenylethanoside product isolated from air-dried fruits of Forsythia suspensa, has been reported to have anti-inflammatory and antioxidant effects. In this study, the anti-inflammatory effects of forsythiaside A on LPS-stimulated BV2 microglia cells and primary microglia cells were investigated. The production of inflammatory mediators TNF-α, IL-1β, NO and PGE2 were detected in this study. NF-κB, nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) expression were detected by western blot analysis. Our results showed that forsythiaside A significantly inhibited LPS-induced inflammatory mediators TNF-α, IL-1β, NO and PGE2 production. LPS-induced NF-κB activation was suppressed by forsythiaside A. Furthermore, forsythiaside A was found to up-regulate the expression of Nrf2 and HO-1. In conclusion, this study demonstrates that forsythiaside A inhibits LPS-induced inflammatory responses in BV2 microglia cells and primary microglia cells through inhibition of NF-κB activation and activation of Nrf2/HO-1 signaling pathway.

Keywords

Forsythiaside A; Inflammatory mediators; LPS; NF-κB; Nrf2.

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