2. Academic Validation
  3. Growing a whole porcine liver organ ex situ for six hours without red blood cells or hemoglobin

Growing a whole porcine liver organ ex situ for six hours without red blood cells or hemoglobin

  • Am J Transl Res. 2016 Jun 15;8(6):2562-74.
Jing Dong 1,  Lingling Xia 2,  Hefang Shen 1,  Congwen Bian 3,  Sujin Bao 4,  Min Zhang 5,  Yiqi Du 6,  Yan Dai 1,  Lijuan Zhao 1,  Yuanhong Xu 5,  Qiru Xiong 3,  Jianjian Xu 7,  Lili Xu 8
Affiliations

Affiliations

  • 1 School of Basic Medical Science, Anhui Medical University 81 Meishan Road, Hefei 230032, Anhui, P. R. China.
  • 2 School of Basic Medical Science, Anhui Medical University81 Meishan Road, Hefei 230032, Anhui, P. R. China; Department of Infectious Diseases, First Affiliated Hospital of Anhui Medical UniversityHefei 230032, P. R. China.
  • 3 Department of Chirurgery, First Affiliated Hospital of Anhui Medical University Hefei 230032, P. R. China.
  • 4 Saint James School of Medicine, Saint Vincent and The Grenadines, First Affiliated Hospital, Anhui Medical University Hefei, P. R. China.
  • 5 Department of Clinical Laboratory, First Affiliated Hospital, Anhui Medical University Hefei, P. R. China.
  • 6 Department of Anesthesiology, Children's Hospital of Anhui Province 39 Eastern Wangjiang Road, Hefei 230002, Anhui, P. R. China.
  • 7 Medical Engineering, Hefei University of Technology Hefei, P. R. China.
  • 8 School of Basic Medical Science, Anhui Medical University81 Meishan Road, Hefei 230032, Anhui, P. R. China; Department of Cell Biology, SUNY Downstate Medical Center760 Parkside Avenue, Brooklyn, New York 11226, USA.
PMID: 27398140
Abstract

Liver transplantation is an effective approach to end-stage liver disease. Shortage of donor liver and increased waiting time for liver transplantation necessitate the development of an organ culture system by which livers can be cultured and maintained ex situ for a prolonged period of time. The aim of this work is to test whether Cell Culture condition in vitro could be used to culture whole livers ex situ without the use of erythrocytes. Twelve castrated male land race/farm young porcine livers were exposed to 30 min warm ischemia and 30 min cold perfusion. Livers were isolated and connected to an Ex situ liver culture system using a standard culture medium RPMI1640 supplied with 10% of fetal bovine serum and sufficient dissolved oxygen under a normothermic condition for 6 hours. Metabolic biomarkers, bile and urea production, hepatic cell viability and histology analysis of biopsies were examined and newly proliferated hepatic cells labeled by BrdU were analyzed after 6 hours ex situ culture. The results from biochemical assays and histology analysis indicate that livers after the organ culture still maintain the full function.

Conclusions: Our data demonstrate that the liver culture system established in this work can be used to culture whole livers ex situ in the absence of erythrocytes.

Keywords

3D culture; BrdU histology analysis; BrdU proliferation assay; Ex situ liver culture; organ growing; oxygen carrier free; warm ischemia; without erythrocytes.

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