2. Cell Cycle/DNA Damage
  3. Nucleoside Antimetabolite/Analog DNA/RNA Synthesis
  4. 5-BrdU

5-BrdU  (Synonyms: BrdU; 5-Bromo-2'-deoxyuridine; BUdR)

Cat. No.: HY-15910 Purity: 99.88%
COA Handling Instructions

Molarity Calculator

5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.

For research use only. We do not sell to patients.

5-BrdU Chemical Structure

5-BrdU Chemical Structure

CAS No. : 59-14-3

Size Price Stock Quantity
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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 In-stock
Solution
10 mM * 1 mL in DMSO USD 33 In-stock
Solid
100 mg USD 30 In-stock
500 mg USD 62 In-stock
1 g USD 100 In-stock
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Customer Review

Based on 55 publication(s) in Google Scholar

5-BrdU Related Antibodies

Top Publications Citing Use of Products

52 Publications Citing Use of MCE 5-BrdU

Proliferation Assay
IF

    5-BrdU purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2018 Jun;15(6):5269-5274.  [Abstract]

    Detection of the effects of CGS 20267 and the MAPK pathway on the proliferation of GC-1 spg cells by BrdU. MAPK, mitogen-activated protein kinase; spg, spermatogonia; BrdU, bromodeoxyuridine.

    5-BrdU purchased from MedChemExpress. Usage Cited in: Obesity (Silver Spring). 2017 Sep;25(9):1532-1539.  [Abstract]

    Representative images for BrdU staining, followed by semiquantitative analysis.

    5-BrdU purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2016 Jun 15;8(6):2562-74.  [Abstract]

    BrdU hepatocyte proliferative assay in ESLC. The representative tissue containing BrdU-positive cells taken after 6 hours of ESLC is shown in (A), and two newly proliferated neighboring positive cells is enlarged and inserted on the right side. (B) shows the experimental negative control and (C) is the analyzed number of BrdU-positive hepatocytes in comparison with control.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.

    In Vitro

    Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    5-BrdU Related Antibodies
    In Vivo

    Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    307.10

    Formula

    C9H11BrN2O5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1C[C@H](N2C(NC(C(Br)=C2)=O)=O)O[C@@H]1CO
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (814.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 2 mg/mL (6.51 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2563 mL 16.2813 mL 32.5627 mL
    5 mM 0.6513 mL 3.2563 mL 6.5125 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 14.29 mg/mL (46.53 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    COA (253 KB) LCMS (125 KB) Elemental Analysis Report (111 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves are obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.2563 mL 16.2813 mL 32.5627 mL 81.4067 mL
    5 mM 0.6513 mL 3.2563 mL 6.5125 mL 16.2813 mL
    DMSO 10 mM 0.3256 mL 1.6281 mL 3.2563 mL 8.1407 mL
    15 mM 0.2171 mL 1.0854 mL 2.1708 mL 5.4271 mL
    20 mM 0.1628 mL 0.8141 mL 1.6281 mL 4.0703 mL
    25 mM 0.1303 mL 0.6513 mL 1.3025 mL 3.2563 mL
    30 mM 0.1085 mL 0.5427 mL 1.0854 mL 2.7136 mL
    40 mM 0.0814 mL 0.4070 mL 0.8141 mL 2.0352 mL
    50 mM 0.0651 mL 0.3256 mL 0.6513 mL 1.6281 mL
    60 mM 0.0543 mL 0.2714 mL 0.5427 mL 1.3568 mL
    80 mM 0.0407 mL 0.2035 mL 0.4070 mL 1.0176 mL
    100 mM 0.0326 mL 0.1628 mL 0.3256 mL 0.8141 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    5-BrdU Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    5-BrdU59-14-3BrdU 5-Bromo-2'-deoxyuridine BUdRNucleoside Antimetabolite/AnalogDNA/RNA SynthesisInhibitorinhibitorinhibit

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