5-BrdU (Synonyms: BrdU; 5-Bromo-2'-deoxyuridine; BUdR)

Name: 5-BrdU
Brand: MedChemExpress
SKU: HY-15910
Rating: 5.0 (57 reviews)

Cat. No.: HY-15910 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.

For research use only. We do not sell to patients.

5-BrdU Chemical Structure

CAS No. : 59-14-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
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100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
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Based on 57 publication(s) in Google Scholar

54 Publications Citing Use of MCE 5-BrdU

Proliferation Assay

: 5-BrdU purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2018 Jun;15(6):5269-5274. [Abstract]; Detection of the effects of CGS 20267 and the MAPK pathway on the proliferation of GC-1 spg cells by BrdU. MAPK, mitogen-activated protein kinase; spg, spermatogonia; BrdU, bromodeoxyuridine.

: 5-BrdU purchased from MedChemExpress. Usage Cited in: Obesity (Silver Spring). 2017 Sep;25(9):1532-1539. [Abstract]; Representative images for BrdU staining, followed by semiquantitative analysis.

: 5-BrdU purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2016 Jun 15;8(6):2562-74. [Abstract]; BrdU hepatocyte proliferative assay in ESLC. The representative tissue containing BrdU-positive cells taken after 6 hours of ESLC is shown in (A), and two newly proliferated neighboring positive cells is enlarged and inserted on the right side. (B) shows the experimental negative control and (C) is the analyzed number of BrdU-positive hepatocytes in comparison with control.

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Biological Activity
Protocol
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References
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Description

5-BrdU (BrdU) is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells.

Cellular Effect

Cell Line	Type	Value	Description	References
L5178Y	IC₅₀	20 μM Compound: BrdUrd	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]Leu. In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]Leu.	[PMID: 11101356]
L5178Y	IC₅₀	26 μM Compound: BrdUrd	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd. In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd.	[PMID: 11101356]

In Vitro

Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile^[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

307.10

Formula

C₉H₁₁BrN₂O₅

CAS No.

59-14-3

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1C[C@H](N2C(NC(C(Br)=C2)=O)=O)O[C@@H]1CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (814.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2 mg/mL (6.51 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2563 mL	16.2813 mL	32.5627 mL
5 mM	0.6513 mL	3.2563 mL	6.5125 mL
10 mM	0.3256 mL	1.6281 mL	3.2563 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 14.29 mg/mL (46.53 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (277 KB) SDS (419 KB)

COA (253 KB) LCMS (125 KB) Elemental Analysis Report (111 KB)

Handling Instructions (2659 KB)

References

[1]. Levkoff LH, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16. [Content Brief]

[2]. Rothaeusler K, et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31 [Content Brief]

Cell Assay
^[1]

Cultures are initially plated at 2000 cells/cm² and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves are obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Levkoff LH, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16. [Content Brief]

[2]. Rothaeusler K, et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.2563 mL	16.2813 mL	32.5627 mL	81.4067 mL
H₂O / DMSO	5 mM	0.6513 mL	3.2563 mL	6.5125 mL	16.2813 mL
DMSO	10 mM	0.3256 mL	1.6281 mL	3.2563 mL	8.1407 mL
	15 mM	0.2171 mL	1.0854 mL	2.1708 mL	5.4271 mL
	20 mM	0.1628 mL	0.8141 mL	1.6281 mL	4.0703 mL
	25 mM	0.1303 mL	0.6513 mL	1.3025 mL	3.2563 mL
	30 mM	0.1085 mL	0.5427 mL	1.0854 mL	2.7136 mL
	40 mM	0.0814 mL	0.4070 mL	0.8141 mL	2.0352 mL
	50 mM	0.0651 mL	0.3256 mL	0.6513 mL	1.6281 mL
	60 mM	0.0543 mL	0.2714 mL	0.5427 mL	1.3568 mL
	80 mM	0.0407 mL	0.2035 mL	0.4070 mL	1.0176 mL
	100 mM	0.0326 mL	0.1628 mL	0.3256 mL	0.8141 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

5-BrdU Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-BrdU59-14-3BrdU 5-Bromo-2'-deoxyuridine BUdRNucleoside Antimetabolite/AnalogDNA/RNA SynthesisInhibitorinhibitorinhibit

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5-BrdU (Synonyms: BrdU; 5-Bromo-2'-deoxyuridine; BUdR)

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5-BrdU Related Antibodies

54 Publications Citing Use of MCE 5-BrdU

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Complete Stock Solution Preparation Table

5-BrdU Related Classifications

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