2. Academic Validation
  3. Effect of FUT3 gene silencing with miRNA on proliferation, invasion and migration abilities of human KATO-III gastric cancer cell line

Effect of FUT3 gene silencing with miRNA on proliferation, invasion and migration abilities of human KATO-III gastric cancer cell line

  • Cell Mol Biol (Noisy-le-grand). 2016 Jun 30;62(7):15-20.
Y-J Cai 1,  X-F Zheng 2,  C-H Lu 3,  Q Jiang 4,  Q Liu 5,  Y-H Xin 6
Affiliations

Affiliations

  • 1 Fourth People's Hospital of Jinan, Taishan Medical College Department of Blood Transfusion Jinan China.
  • 2 Fourth People's Hospital of Jinan, Taishan Medical College Clinical Laboratory Jinan China.
  • 3 Fourth People's Hospital of Jinan, Taishan Medical College Nursing Department Jinan China.
  • 4 Jiaozhou Central Hospital of Qingdao Clinical Laboratory Jiaozhou China.
  • 5 China National Heavy Duty Truck Group Corporation Hospital Jinan China.
  • 6 Fourth People's Hospital of Jinan, Taishan Medical College Department of Blood Transfusion Jinan China xyhlq@163.com.
PMID: 27453266
Abstract

This study investigated the effects of FUT3 gene expression inhibition with miRNA on the proliferation, invasion and migration abilities of KATO-III cells. KATO-III cells were transfected with plasmid pcDNA™6.2-GW/EmGFP-FUT3-miR(FUT3-miRNA) and negative control plasmid in mediation of Liposome, respectively, using untransfected cells as blank controls. Forty-eight hours after transfection, FUT3 mRNA levels were tested by RT-PCR. Levels of sLeA proteins were assayed by Western blot. The effects of FUT3-miRNA on the proliferation, invasion and migration of KATO-III cells were determined by CCK8 testing and Transwell assays, respectively. Results indicate that the transfection of FUT3-miRNA may down-regulate sLeA protein expression on the surface of KATO-III cells, and significantly inhibit cell proliferation (p<0.05). As compared to the negative and blank control groups, the number of invasion and migration cells in the FUT3-miRNA group decreased significantly (each p<0.05). Experimental results indicate that the miRNA expression vector which targets the FUT3 gene can effectively inhibit the proliferation, migration and invasion abilities of KATO-III cells.

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