2. Academic Validation
  3. Vanillin attenuates negative effects of ultraviolet A on the stemness of human adipose tissue-derived mesenchymal stem cells

Vanillin attenuates negative effects of ultraviolet A on the stemness of human adipose tissue-derived mesenchymal stem cells

  • Food Chem Toxicol. 2016 Oct;96:62-9. doi: 10.1016/j.fct.2016.07.023.
Sang Yeol Lee 1,  See-Hyoung Park 2,  Mi Ok Kim 3,  Inhwan Lim 4,  Mingyeong Kang 3,  Sae Woong Oh 3,  Kwangseon Jung 5,  Dong Gyu Jo 4,  Il-Hoon Cho 6,  Jongsung Lee 7
Affiliations

Affiliations

  • 1 Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, 131-20 Gyunggi Do, Republic of Korea.
  • 2 Department of Bio and Chemical Engineering, Hongik University, 300-16 Sejong City, Republic of Korea.
  • 3 Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
  • 4 Labatory of Molecular and Cellular Biology, School of Pharmacy, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea.
  • 5 Skincure Life Science Institute, Seongnam City, 132-16 Gyunggi Do, Republic of Korea.
  • 6 Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam City, 131-35 Gyunggi Do, Republic of Korea. Electronic address: chiuchun0716@gmail.com.
  • 7 Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, 164-19 Gyunggi Do, Republic of Korea. Electronic address: bioneer@skku.edu.
PMID: 27470612 DOI: 10.1016/j.fct.2016.07.023
Abstract

Ultraviolet A (UVA) irradiation induces various changes in Cell Biology. The objective of this study was to determine the effect of vanillin on UVA irradiation-induced damages in the stemness properties of human adipose tissue-derived mesenchymal stem cells (hAMSCs). UVA-antagonizing mechanisms of vanillin were also examined. The results revealed that vanillin attenuated UVA-induced reduction of the proliferative potential and stemness of hAMSCs evidenced by increased proliferative activity in BrdU incorporation assay and upregulation of stemness-related genes (OCT4, NANOG and SOX2) in response to vanillin treatment. UVA-induced reduction in mRNA level of hypoxia-inducible factor (HIF)-1α was significantly recovered by vanillin. In addition, the antagonizing effect of vanillin on UVA was found to be mediated by reduced production of PGE2 through inhibiting JNK and p38 MAPK. Taken together, these findings showed that vanillin could improve the reduced stemness of hAMSCs induced by UVA. The effect of vanillin is mediated by upregulating HIF-1α via inhibiting PGE2-cAMP signaling. Therefore, vanillin might be used as an antagonizing agent to mitigate the effects of UVA.

Keywords

HIF; Human adipose tissue-derived mesenchymal stem cells; PGE(2); Stemness gene; UVA toxicity; Vanillin.

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