2. Academic Validation
  3. Activin A/Smads signaling pathway negatively regulates Oxygen Glucose Deprivation-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells

Activin A/Smads signaling pathway negatively regulates Oxygen Glucose Deprivation-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells

  • Biochem Biophys Res Commun. 2016 Nov 18;480(3):355-361. doi: 10.1016/j.bbrc.2016.10.050.
Long-Xing Xue 1,  Zhong-Hang Xu 2,  Jiao-Qi Wang 1,  Yang Cui 1,  Hong-Yu Liu 1,  Wen-Zhao Liang 1,  Qiu-Ye Ji 3,  Jin-Ting He 1,  Yan-Kun Shao 1,  Jing Mang 4,  Zhong-Xin Xu 5
Affiliations

Affiliations

  • 1 Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
  • 2 Clinical Medicine of Norman Bethune Medical Department, Jilin University, Changchun 130012, Jilin Province, China.
  • 3 Research Center, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
  • 4 Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China. Electronic address: mangjing@jlu.edu.cn.
  • 5 Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China. Electronic address: xuzhongxin999@aliyun.com.
PMID: 27769861 DOI: 10.1016/j.bbrc.2016.10.050
Abstract

Activin A (Act A), a member of the transforming growth factor-beta (TGF-β), reduces neuronal Apoptosis during cerebral ischemia through Act A/Smads signaling pathway. However, little is known about the effect of Act A/Smads pathway on Autophagy in neurons. Here, we found that oxygen-glucose deprivation (OGD)-induced Autophagy was suppressed by exogenous Act A in a concentration-dependent manner and enhanced by Act A/Smads pathway inhibitor (ActRIIA-Ab) in neuronal PC12 cells. These results indicate that Act A/Smads pathway negatively regulates Autophagy in OGD-treated PC12 cells. In addition, we found that c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways are involved in the OGD-induced Autophagy. The activation of JNK and p38 MAPK pathways in OGD-treated PC12 cells was suppressed by exogenous Act A and enhanced by ActRIIA-Ab. Together, our results suggest that Act A/Smads signaling pathway negatively regulates OGD-induced Autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells.

Keywords

Activin A/Smads; Autophagy; JNK; Oxygen Glucose Deprivation (OGD); p38.

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