2. Academic Validation
  3. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells

Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells

  • Methods Mol Biol. 2017;1534:111-119. doi: 10.1007/978-1-4939-6670-7_10.
Konstantinos Evangelou 1,  Vassilis G Gorgoulis 2 3 4
Affiliations

Affiliations

  • 1 Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, Athens, 11527, Greece.
  • 2 Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, Athens, 11527, Greece. vgorg@med.uoa.gr.
  • 3 Biomedical Research Foundation, Academy of Athens, Athens, Greece. vgorg@med.uoa.gr.
  • 4 Faculty of Biology, Medicine and Health Manchester Cancer Research Centre, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK. vgorg@med.uoa.gr.
PMID: 27812872 DOI: 10.1007/978-1-4939-6670-7_10
Abstract

The Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions.

Keywords

Archival material; Fresh material; Histochemical stain; Lipofuscin; Paraffin; Senescence; Sudan-Black-B.

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