2. Academic Validation
  3. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes

Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes

  • Exp Ther Med. 2016 Nov;12(5):3488-3494. doi: 10.3892/etm.2016.3784.
Shu-Guang Gao 1,  Yang Yu 2,  Chao Zeng 1,  Shi-Tao Lu 2,  Jian Tian 1,  Chao Cheng 3,  Liang-Jun Li 4,  Guang-Hua Lei 5
Affiliations

Affiliations

  • 1 Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
  • 2 Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China; Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.
  • 3 Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China; Department of Orthopedics, Yiyang Central Hospital, Yiyang, Hunan 413000, P.R. China.
  • 4 Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China; Department of Orthopedics, Changsha Central Hospital, Changsha, Hunan 410004, P.R. China.
  • 5 Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China; Research Center of Clinical Skills of Joint Surgery of Hunan Province, Changsha, Hunan 410008, P.R. China.
PMID: 27882184 DOI: 10.3892/etm.2016.3784
Abstract

The aim of the present study was to investigate the effects of phosphorylated osteopontin (p-OPN) on Apoptosis and pro-inflammatory cytokine expression in human knee osteoarthritis (OA) chondrocytes. Human knee OA chondrocytes obtained from patients who underwent total knee arthroplasty were treated with p-OPN, OPN or buffer. Reverse transcription quantitative-polymerase chain reaction (RT-qPCR) and western blot analysis were used to assess the expression levels of proinflammatory factors, including interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6 and nuclear factor (NF)-κB. Apoptosis of human knee OA chondrocytes was detected by Annexin V-fluorescein isothiocyanate/propidium iodide flow cytometry. Compared with the controls, chondrocytes treated with OPN exhibited higher mRNA and protein expression levels of proinflammatory factors (IL-1β, TNF-α, IL-6 and NF-κB), and a higher percentage of apoptotic chondrocytes. Furthermore, chondrocytes treated with p-OPN exhibited the highest mRNA and protein expression levels of proinflammatory factors (IL-1β, TNF-α, IL-6, NF-κB) and the highest percentage of apoptotic chondrocytes. p-OPN induces chondrocyte Apoptosis and proinflammatory factor release, which suggests that p-OPN may contribute to OA pathogenesis, and inhibition of p-OPN may provide a novel effective strategy to slow or halt OA progression.

Keywords

apoptosis; chondrocyte; osteoarthritis; osteopontin; phosphorylation.

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