1. Academic Validation
  2. Triolein reduces MMP-1 upregulation in dermal fibroblasts generated by ROS production in UVB-irradiated keratinocytes

Triolein reduces MMP-1 upregulation in dermal fibroblasts generated by ROS production in UVB-irradiated keratinocytes

  • J Dermatol Sci. 2017 Feb;85(2):124-130. doi: 10.1016/j.jdermsci.2016.11.010.
Gustavo J Leirós 1 Ana Gabriela Kusinsky 2 María Eugenia Balañá 3 Karin Hagelin 4
Affiliations

Affiliations

  • 1 Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: gleiros@fundacioncassara.org.ar.
  • 2 Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: gkusinsky@fundacioncassara.org.ar.
  • 3 Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: mbalana@fundacioncassara.org.ar.
  • 4 Fundación Pablo Cassará-Instituto de Ciencia y Tecnología Dr. César Milstein, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Saladillo 2468 C1440FFX, Ciudad de Buenos Aires, Argentina. Electronic address: khagelin@fundacioncassara.org.ar.
Abstract

Background: Cytokine production and oxidative stress generated by ultraviolet radiation B (UVB) skin exposure are main factors of skin photoaging. Interleukin-6 (IL-6) produced by irradiated keratinocytes is proposed to have a role in metalloproteinases (MMPs) expression activation in dermal fibroblasts.

Objectives: We examined the effect of triolein treatment of UVB-irradiated keratinocytes on MMP1 (interstitial collagenase) expression response of dermal fibroblasts. We assayed UVB-irradiated keratinocytes soluble signals, mainly IL-6 and Reactive Oxygen Species (ROS).

Methods: IL-6 expression and ROS generation were assayed in UVB-irradiated keratinocytes. MMP1 mRNA expression response was assayed in fibroblasts grown in keratinocytes conditioned medium. We evaluated the effect of treating keratinocytes with triolein on IL-6 expression and ROS generation in keratinocytes, and MMP1 expression in fibroblasts.

Results: The irradiation of epidermal cells with sublethal UVB doses increased IL-6 expression and ROS generation. Conditioned culture medium collected from keratinocytes was used to culture dermal fibroblasts. MMP1 mRNA expression increase was observed in fibroblasts cultured in medium collected from UVB-irradiated keratinocytes. Triolein treatment reduced the IL-6 expression and ROS generation in keratinocytes and this effect was reflected in downregulation of MMP1 expression in fibroblasts.

Conclusions: Triolein reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes. It seems to exert an anti-inflammatory and anti-oxidative stress effect on irradiated keratinocytes that in turn reduces MMP1 expression in dermal fibroblasts. Collectively, these results indicate that triolein could act as a photoprotective agent.

Keywords

MMP1; ROS; Triolein IL-6; UVB.

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