2. Academic Validation
  3. Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor

Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor

  • Cell. 2017 Jan 12;168(1-2):224-238.e10. doi: 10.1016/j.cell.2016.11.042.
Yongjie Wei 1,  Wei-Chung Chiang 2,  Rhea Sumpter Jr 2,  Prashant Mishra 3,  Beth Levine 4
Affiliations

Affiliations

  • 1 Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA.
  • 2 Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA.
  • 3 Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA.
  • 4 Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA; Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA. Electronic address: beth.levine@utsouthwestern.edu.
PMID: 28017329 DOI: 10.1016/j.cell.2016.11.042
Abstract

The removal of unwanted or damaged mitochondria by Autophagy, a process called Mitophagy, is essential for key events in development, cellular homeostasis, tumor suppression, and prevention of neurodegeneration and aging. However, the precise mechanisms of Mitophagy remain uncertain. Here, we identify the inner mitochondrial membrane protein, prohibitin 2 (PHB2), as a crucial Mitophagy receptor involved in targeting mitochondria for autophagic degradation. PHB2 binds the autophagosomal membrane-associated protein LC3 through an LC3-interaction region (LIR) domain upon mitochondrial depolarization and proteasome-dependent outer membrane rupture. PHB2 is required for Parkin-induced Mitophagy in mammalian cells and for the clearance of paternal mitochondria after embryonic fertilization in C. elegans. Our findings pinpoint a conserved mechanism of eukaryotic Mitophagy and demonstrate a function of prohibitin 2 that may underlie its roles in physiology, aging, and disease.

Keywords

autophagy; mitophagy; prohibitin 2.

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