2. Academic Validation
  3. Potent Antiarthritic Properties of Phloretin in Murine Collagen-Induced Arthritis

Potent Antiarthritic Properties of Phloretin in Murine Collagen-Induced Arthritis

  • Evid Based Complement Alternat Med. 2016;2016:9831263. doi: 10.1155/2016/9831263.
Shun-Ping Wang 1,  Shih-Chao Lin 2,  Shiming Li 3,  Ya-Hsuan Chao 4,  Guang-Yuh Hwang 5,  Chi-Chen Lin 6
Affiliations

Affiliations

  • 1 Department of Life Science, Tunghai University, Taichung, Taiwan; Department of Orthopaedics, Taichung Veterans General Hospital, Taichung, Taiwan.
  • 2 Ph.D. Program in Medical Biotechnology, National Chung Hsing University, Taichung, Taiwan; SRI International, Harrisonburg, VA, USA.
  • 3 Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization and Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang, Hubei, China.
  • 4 Institute of Biomedical Science, National Chung Hsing University, Taichung, Taiwan.
  • 5 Department of Life Science, Tunghai University, Taichung, Taiwan.
  • 6 Institute of Biomedical Science, National Chung Hsing University, Taichung, Taiwan; Department of Biotechnology, Asia University, Taichung, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
PMID: 28044086 DOI: 10.1155/2016/9831263
Abstract

In the exploration of potential therapeutic agents for rheumatoid arthritis (RA), DBA/1J mice are used as the RA model of collagen-induced arthritis (CIA). Phloretin, a flavonoid compound extracted from Prunus mandshurica, has been found to exhibit anti-inflammatory activity, making it a potential candidate for treatment of RA. The objective of this study was to evaluate the therapeutic effects of phloretin on CIA mice. CIA mice were dosed daily with phloretin at either 50 or 100 mg/kg among two treatment groups. CIA treated mice showed mitigation of clinical symptoms of RA in addition to reduced inflammation of hind-limbs compared to mice who did not receive phloretin. Histological analysis showed that phloretin suppressed the severity of RA and effectively mitigated joint inflammation and cartilage- and bone-destruction via reducing proinflammatory cytokine productions (TNF-α, IL-6, IL-1β, and IL-17). This was at least partially mediated by causing inadequate splenocyte activation and proliferation. Moreover, phloretin-treated CIA mice showed decreased oxidative stress and diminished levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in paw tissues as well as reduced productivity of anti-collagen Antibodies in serum. We have concluded that phloretin could be a potent and effective antiarthritis agent, demonstrating anti-inflammatory, antioxidative, and immunomodulatory effects in CIA mice.

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