2. Academic Validation
  3. Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells

Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells

  • Biomater Sci. 2017 Feb 28;5(3):511-522. doi: 10.1039/c6bm00856a.
Juan Lv 1,  Xuefang Hao 1,  Qian Li 1,  Mary Akpanyung 2,  Abdelilah Nejjari 2,  Agnaldo Luis Neve 2,  Xiangkui Ren 3,  Yakai Feng 4,  Changcan Shi 5,  Wencheng Zhang 6
Affiliations

Affiliations

  • 1 School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China.
  • 2 School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn.
  • 3 School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Yaguan Road 135, Tianjin 300350, China.
  • 4 School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China. yakaifeng@tju.edu.cn and Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China and Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Yaguan Road 135, Tianjin 300350, China and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Weijin Road 92, Tianjin 300072, China.
  • 5 Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325011, China and Wenzhou Institute of Biomaterials and Engineering, CNITECH, CAS, Wenzhou, Zhejiang 325011, China.
  • 6 Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin 300162, China.
PMID: 28094352 DOI: 10.1039/c6bm00856a
Abstract

In this work, a biodegradable star-shaped copolymer poly(lactide-co-3(S)-methyl-morpholine-2,5-dione)6 (Star-(PLMD)6) was synthesized via ring-opening polymerization (ROP), and subsequently a gene carrier Star-PLMD-g-PEI-g-PEG-CREDVW was prepared by grafting polyethyleneimine (PEI), polyethylene glycol (PEG) and targeting peptide REDV onto Star-(PLMD)6. This gene carrier could form stable micelles to condense pEGFP-ZNF580 through electrostatic interaction. The resulting complexes were biocompatible and showed high efficiency in gene delivery. In addition, these complexes exhibited high selectivity for endothelial cells (ECs), high transfection efficiency and enhanced migration of ECs. The protein level of ZNF580 expression was significantly high (up to 85%), while the control group was only 51%. This combination of degradability, targeting ligand and star-structure strategy exhibits a significant advantage in transfection efficiency and migration of ECs.

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