2. Academic Validation
  3. PPPDE1 is a novel deubiquitinase belonging to a cysteine isopeptidase family

PPPDE1 is a novel deubiquitinase belonging to a cysteine isopeptidase family

  • Biochem Biophys Res Commun. 2017 Jun 24;488(2):291-296. doi: 10.1016/j.bbrc.2017.04.161.
Xingwang Xie 1,  Xueyan Wang 2,  Dong Jiang 3,  Jianghua Wang 2,  Ran Fei 2,  Xu Cong 2,  Lai Wei 2,  Yu Wang 4,  Hongsong Chen 5
Affiliations

Affiliations

  • 1 Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, China; Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • 2 Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, China.
  • 3 Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
  • 4 Chinese Center for Disease Control and Prevention, Beijing 102206, China. Electronic address: wangyu@chinacdc.cn.
  • 5 Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, China. Electronic address: chenhongsong2999@163.com.
PMID: 28483520 DOI: 10.1016/j.bbrc.2017.04.161
Abstract

Ubiquitinlation of proteins is prevalent and important in both normal and pathological cellular processes. Deubiquitinating Enzymes (DUBs) can remove the ubiquitin tags on substrate proteins and dynamically regulate the ubiquitination process. The PPPDE family proteins were predicted to be a novel class of deubiquitinating peptidase, but this has not yet been experimentally proved. Here we validated the deubiquitinating activity of PPPDE1 and revealed its isopeptidase activity against ubiquitin conjugated through Lys 48 and Lys 63. We also identified ribosomal protein S7, RPS7, as a substrate protein of PPPDE1. Moreover, PPPDE1 could mediate the ubiquitin chain editing of RPS7, deubiquitinating Lys 48-linked ubiquitination, and finally stabilize RPS7 proteins. Taken together, we report that PPPDE1 is a novel Deubiquitinase that belongs to a cysteine isopeptidase family.

Keywords

Cysteine isopeptidase; DUBs; Deubiquitinase; PPPDE1; RPS7.

Figures