2. Academic Validation
  3. IL-17RB enhances thyroid cancer cell invasion and metastasis via ERK1/2 pathway-mediated MMP-9 expression

IL-17RB enhances thyroid cancer cell invasion and metastasis via ERK1/2 pathway-mediated MMP-9 expression

  • Mol Immunol. 2017 Oct;90:126-135. doi: 10.1016/j.molimm.2017.06.034.
Lei Ren 1,  Yapei Xu 2,  Cong Liu 3,  Shoujun Wang 1,  Guijun Qin 4
Affiliations

Affiliations

  • 1 Department of Endocrinology, The First Affiliated Hospital of ZhengZhou University, Zhengzhou 450002, China.
  • 2 Department of Nephrology, The Third People's Hospital of ZhengZhou, Zhengzhou 450000, China.
  • 3 Department of Endocrinology, ShengJing Hospital of China Medical University, Shenyang 110022, China.
  • 4 Department of Endocrinology, The First Affiliated Hospital of ZhengZhou University, Zhengzhou 450002, China. Electronic address: qinguijunn@163.com.
PMID: 28715683 DOI: 10.1016/j.molimm.2017.06.034
Abstract

IL-17RB, a member of the IL-17 Receptor family that can be activated by IL-17B, has been proved to be involved in inflammatory diseases and cancers. However, the function of IL-17RB in thyroid Cancer is still unknown. In this study, IL-17RB expression in thyroid Cancer cell lines and tissues was examined by Real-Time PCR and western blot. The effects of IL-17RB on cell invasion and migration were determined by in vitro invasion and migration assays, while the effects of IL-17RB on cell metastasis were analyzed by in vivo experiments. The results showed that IL-17RB expression was upregulated in both thyroid Cancer cells and tissues. IL-17B dose-dependently promoted the invasion, growth and migration of thyroid Cancer cells, whereas knockdown of IL-17RB attenuated the effects of IL-17B in vitro. Moreover, IL-17RB was involved in the metastasis and growth of thyroid Cancer cells in vivo. In addition, IL-17RB induced ERK1/2 activation and increased MMP-9 expression in vitro and in vivo. Inhibition of ERK1/2 pathway blocked the IL-17RB-mediated thyroid Cancer cell invasion and MMP-9 expression. Together, our findings demonstrate that IL-17RB can enhance thyroid Cancer cell invasion and metastasis via ERK1/2 pathway-mediated MMP-9 expression, suggesting that IL-17RB may act as a potential therapeutic target for thyroid Cancer therapy.

Keywords

ERK1/2 pathway; IL-17B; IL-17RB; Invasion; Metastasis; Thyroid cancer.

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