2. Academic Validation
  3. Involvement of NLRP10 in IL-1α induction of oral epithelial cells by periodontal pathogens

Involvement of NLRP10 in IL-1α induction of oral epithelial cells by periodontal pathogens

  • Innate Immun. 2017 Oct;23(7):569-577. doi: 10.1177/1753425917722610.
Seok-Joo Lee 1,  Bong-Kyu Choi 1 2
Affiliations

Affiliations

  • 1 1 Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Korea.
  • 2 2 Dental Research Institute, Seoul National University, Seoul, Korea.
PMID: 28766990 DOI: 10.1177/1753425917722610
Abstract

This study investigated the pathogenesis of periodontitis and the role of nucleotide-binding oligomerization domain-like receptor protein 10 (NLRP10). The human oral epithelial cell line HOK-16B was infected with two periodontal pathogens, Tannerella forsythia and Fusobacterium nucleatum, at various MOIs. RT-PCR and immunoblotting demonstrated that Infection increased mRNA and protein expression of NLRP10, respectively. The siRNA-mediated NLRP10 knockdown significantly reduced IL-1α expression and secretion. Both bacteria induced phosphorylation of ERK, JNK and p38 MAP kinases in HOK-16B cells. NLRP10 knockdown impaired ERK phosphorylation only. ERK inhibition significantly decreased the expression of T. forsythia- and F. nucleatum-induced IL-1α. Our data suggest that NLRP10 is involved in activating the ERK signalling pathway in HOK-16B cells infected with T. forsythia and F. nucleatum. This pathway likely augments the pro-inflammatory cytokine IL-1α levels, which may play a critical role in periodontitis.

Keywords

Fusobacterium nucleatum; IL-1α; NLRP10; Tannerella forsythia; periodontitis.

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