2. Academic Validation
  3. Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

  • Eur J Clin Microbiol Infect Dis. 2017 Dec;36(12):2379-2383. doi: 10.1007/s10096-017-3070-0.
R Alvarez-Marin 1 2 3 4,  M Aires-de-Sousa 5,  P Nordmann 1 2 3 6,  N Kieffer 1 2 3,  L Poirel 7 8 9
Affiliations

Affiliations

  • 1 Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, rue Albert Gockel 3, 1700, Fribourg, Switzerland.
  • 2 French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland.
  • 3 National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland.
  • 4 Hospital Universitario Virgen del Rocio y Virgen Macarena, Seville, Spain.
  • 5 Escola Superior de Saúde da Cruz Vermelha Portuguesa, Lisbon, Portugal.
  • 6 University of Lausanne and University Hospital Center, Lausanne, Switzerland.
  • 7 Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, rue Albert Gockel 3, 1700, Fribourg, Switzerland. laurent.poirel@unifr.ch.
  • 8 French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland. laurent.poirel@unifr.ch.
  • 9 National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland. laurent.poirel@unifr.ch.
PMID: 28825186 DOI: 10.1007/s10096-017-3070-0
Abstract

Multidrug-resistant (MR) Gram-negative (GN) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic Antibiotics. Well-tolerated antiseptics, such as octenidine dihydrochloride (OCT), may be a very useful tool in Infection control to reduce the dissemination of MRGN. This study aimed to investigate the bactericidal activity of OCT against international epidemic clones of MRGN. A set of five different species (Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa) was studied to prove OCT efficacy without organic load, under "clean conditions" (0.3 g/L albumin) and under "dirty conditions" (3 g/L albumin + 3 mL/L defibrinated sheep blood), according to an official test norm (EN13727). We used five clonally unrelated isolates per species, including a susceptible wild-type strain, and four MRGN isolates, corresponding to either the 3MRGN or 4MRGN definition of multidrug resistance. A contact time of 1 min was fully effective for all isolates by using different OCT concentrations (0.01% and 0.05%), with a Bacterial reduction factor of >5 log10 systematically observed. Growth kinetics were determined with two different wild-type strains (A. baumannii and K. pneumoniae), proving a time-dependent efficacy of OCT. These results highlight that OCT may be extremely useful to eradicate emerging highly resistant Gram-negative pathogens associated with nosocomial infections.

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