2. Academic Validation
  3. Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells

Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells

  • In Vivo. 2018 Mar-Apr;32(2):279-285. doi: 10.21873/invivo.11235.
Tsung-Hsien Yen 1,  Chia-Ling Hsieh 2,  Tsu-Te Liu 3,  Chih-Sheng Huang 4 5,  Yen-Chung Chen 6,  Yao-Chen Chuang 7,  Song-Shei Lin 8,  Fei-Ting Hsu 9 10 11 12
Affiliations

Affiliations

  • 1 Department of Radiology, Cheng Ching General Hospital, Taichung, Taiwan, R.O.C.
  • 2 The Ph.D. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan, R.O.C.
  • 3 Division of Gastroenterology, Department of Internal Medicine, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C.
  • 4 Department of Surgery, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C.
  • 5 Department of Surgery, School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
  • 6 Department of Pathology, National Yang-Ming University Hospital, Yilan, Taiwan, R.O.C.
  • 7 Kiang Wu Nursing College of Macau, Macau SAR, P.R. China.
  • 8 Department of Medical Imaging and Radiological Sciences, Central-Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. sslin@ctust.edu.tw sakiro920@gmail.com.
  • 9 Department of Radiology, School of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C. sslin@ctust.edu.tw sakiro920@gmail.com.
  • 10 Department of Medical Imaging, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.
  • 11 Research Center of Translational Imaging, College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C.
  • 12 Translational Laboratory, Department of Medical Research, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.
PMID: 29475910 DOI: 10.21873/invivo.11235
Abstract

The goal of the present study was to investigate Anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting Enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active Caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic Apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro.

Keywords

Glioblastoma; NF-ĸB; amentoflavone; apoptosis.

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