Amentoflavone (Synonyms: Didemethyl-ginkgetin)

Name: Amentoflavone
Brand: MedChemExpress
SKU: HY-N0662
Rating: 5.0 (9 reviews)

Cat. No.: HY-N0662 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

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Amentoflavone (Didemethyl-ginkgetin) is a potent and orally active GABA(A) negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone induces apoptosis and cell cycle arrest at sub-G1 phase.

For research use only. We do not sell to patients.

Amentoflavone Chemical Structure

CAS No. : 1617-53-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 71	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 71	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 470	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Amentoflavone:

Amentoflavone (Standard) Get quote

9 Publications Citing Use of MCE Amentoflavone

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	8.36 μM Compound: 10	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
Calu-1	IC₅₀	> 100 μM Compound: 6	Cytotoxicity against human Calu1 cells after 3 days Cytotoxicity against human Calu1 cells after 3 days	[PMID: 10843573]
Fibroblast	IC₅₀	1.8 μM Compound: 4	Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs	[PMID: 18029185]
HCT-15	IC₅₀	> 50 μM Compound: 8	Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HeLa	IC₅₀	> 100 μM Compound: 6	Cytotoxicity against human HeLa cells after 3 days Cytotoxicity against human HeLa cells after 3 days	[PMID: 10843573]
HeLa	IC₅₀	46.4 μM Compound: amentoflavone	Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay	[PMID: 26722868]
HeLa	IC₅₀	5.3 μM Compound: 7	Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay	[PMID: 29475587]
HepG2	IC₅₀	> 50 μM Compound: 8	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HepG2	IC₅₀	9.13 μM Compound: 10	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
HL-60	IC₅₀	> 50 μM Compound: 8	Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HL-60	IC₅₀	51.2 μM Compound: 7	Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay	[PMID: 29475587]
Huh-7	CC₅₀	> 50 μM Compound: 12	Cytotoxicity against human Huh7.5.1 cells by MTT assay Cytotoxicity against human Huh7.5.1 cells by MTT assay	[PMID: 22445328]
Jurkat	IC₅₀	> 50 μM Compound: 8	Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
K562	IC₅₀	> 100 μM Compound: 6	Cytotoxicity against human K562 cells after 3 days Cytotoxicity against human K562 cells after 3 days	[PMID: 10843573]
MCF7	IC₅₀	75.4 μM Compound: 4	Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay	[PMID: 34875389]
MGC-803	IC₅₀	9.86 μM Compound: 10	Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
Monocyte	IC₅₀	3 μM Compound: amentoflavone	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
Neutrophil	IC₅₀	0.75 μM Compound: 7	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate	[PMID: 29381070]
Neutrophil	IC₅₀	1.01 μM Compound: 7	Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase	[PMID: 29381070]
PBMC	IC₅₀	35 μM Compound: 1	Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method	[PMID: 9322359]
Raji	IC₅₀	> 100 μM Compound: 6	Cytotoxicity against human Raji cells after 3 days Cytotoxicity against human Raji cells after 3 days	[PMID: 10843573]
SW480	IC₅₀	111 μM Compound: 9	Cytotoxicity against human SW480 cells after 72 hrs by MTT assay Cytotoxicity against human SW480 cells after 72 hrs by MTT assay	[PMID: 15787435]
T-24	IC₅₀	10.67 μM Compound: 10	Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
THP-1	IC₅₀	> 50 μM Compound: 8	Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
U-937	IC₅₀	> 50 μM Compound: 8	Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
Vero	IC₅₀	> 100 μM Compound: 6	Cytotoxicity against african green monkey Vero cells after 3 days Cytotoxicity against african green monkey Vero cells after 3 days	[PMID: 10843573]
WISH	IC₅₀	> 100 μM Compound: 6	Cytotoxicity against human Wish cells after 3 days Cytotoxicity against human Wish cells after 3 days	[PMID: 10843573]

In Vitro

Amentoflavone (1-60 μM) inhibits the production of nitric oxide in a concentration-dependent manner in RAW 264.7 cells^[2].
? Amentoflavone (50-200 μM) inhibits the viability of U-87 MG cells with IC₅₀ value of 100 μM at 48 h^[3].
? Amentoflavone (0, 50, 100 μM; 48 h) induces apoptosis and cell cycle arrest at sub-G1 phase^[3].
? Amentoflavone (0, 50, 100 μM; 48 h) inhibits NF-?B activation and decreases the expression of MCL1 and C-FLIP protein in U-87 MG cells^[3].
? Amentoflavone (0-32 μg/ml) shows antibacterial activity with MICs of 8, 4, 32, 8, 16, 8 μg/ml for E. faecium ATCC 19434, S. aureus ATCC 25923, S. mutans ATCC 3065, E. coli O-157 ATCC 25922, E. coli ATCC 43895, P. aeruginosa ATCC 27853, respectively^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	U-87 MG cells
Concentration:	0, 50, 75, 100, 200 µM
Incubation Time:	48 h
Result:	Significantly inhibited the viability of U-87 MG cells by 23-71% with an IC₅₀ value of 100 µM at 48 h.

Apoptosis Analysis^[3]

Cell Line:	U-87 MG cells
Concentration:	0, 50, 100 µM
Incubation Time:	48 h
Result:	Significantly induced the accumulation of cells in the sub-G1 population and increased the level of active caspase-3 by 14-52% and 24-42%, respectively, and significantly triggered the loss of Ψm and the expression of active caspase-8 by 23-53% and 25-50%, respectively.

Western Blot Analysis^[3]

Cell Line:	U-87 MG cells
Concentration:	0, 50, 100 µM
Incubation Time:	48 h
Result:	Significantly reduced NF-ĸB activation in a dose-dependent manner by 25-87% and reduced protein expression of MCL1 and C-FLIP by 50-80% and 38-57%, respectively.

In Vivo

Amentoflavone (25 mg/kg; p.o.; once a day for 3 consecutive days) shows neuroprotective role in epilepsy via anti-inflammatory effects in mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5-6 weeks, 28-32 g, kunming mice^[1]
Dosage:	25 mg/kg
Administration:	P.o.; once a day for 3 consecutive days
Result:	Inhibited activation and nuclear translocation of NF-κB subunits p65, decreased IL-6 and IL-1β production and significantly decreased NO and prostaglandin E2 production.

Molecular Weight

538.46

Formula

C₃₀H₁₈O₁₀

CAS No.

1617-53-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4O)C(O)=CC(O)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (232.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8571 mL	9.2857 mL	18.5715 mL
5 mM	0.3714 mL	1.8571 mL	3.7143 mL
10 mM	0.1857 mL	0.9286 mL	1.8571 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.64 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (288 KB) SDS (393 KB)

COA (261 KB) HNMR (299 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang Z, et al. Amentoflavone protects hippocampal neurons: anti-inflammatory, antioxidative, and antiapoptotic effects. Neural Regen Res. 2015 Jul;10(7):1125-33. [Content Brief]

[2]. Woo ER, et al. Amentoflavone inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB activation in macrophages. Pharmacol Res. 2005 Jun;51(6):539-46. [Content Brief]

[3]. Yen TH, et al. Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells. In Vivo. 2018 Mar-Apr;32(2):279-285. [Content Brief]

[4]. Hwang JH, et al. Antibacterial effect of amentoflavone and its synergistic effect with antibiotics. J Microbiol Biotechnol. 2013;23(7):953-8. [Content Brief]

[5]. Hanrahan JR, et al. Semisynthetic preparation of amentoflavone: A negative modulator at GABA(A) receptors. Bioorg Med Chem Lett. 2003 Jul 21;13(14):2281-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8571 mL	9.2857 mL	18.5715 mL	46.4287 mL
	5 mM	0.3714 mL	1.8571 mL	3.7143 mL	9.2857 mL
	10 mM	0.1857 mL	0.9286 mL	1.8571 mL	4.6429 mL
	15 mM	0.1238 mL	0.6190 mL	1.2381 mL	3.0952 mL
	20 mM	0.0929 mL	0.4643 mL	0.9286 mL	2.3214 mL
	25 mM	0.0743 mL	0.3714 mL	0.7429 mL	1.8571 mL
	30 mM	0.0619 mL	0.3095 mL	0.6190 mL	1.5476 mL
	40 mM	0.0464 mL	0.2321 mL	0.4643 mL	1.1607 mL
	50 mM	0.0371 mL	0.1857 mL	0.3714 mL	0.9286 mL
	60 mM	0.0310 mL	0.1548 mL	0.3095 mL	0.7738 mL
	80 mM	0.0232 mL	0.1161 mL	0.2321 mL	0.5804 mL
	100 mM	0.0186 mL	0.0929 mL	0.1857 mL	0.4643 mL

Amentoflavone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Amentoflavone (Synonyms: Didemethyl-ginkgetin)

Customer Review

Other Forms of Amentoflavone:

9 Publications Citing Use of MCE Amentoflavone

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

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Complete Stock Solution Preparation Table

Amentoflavone Related Classifications

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Amentoflavone (Synonyms: Didemethyl-ginkgetin)

Customer Review

Other Forms of Amentoflavone:

Amentoflavone Related Antibodies

9 Publications Citing Use of MCE Amentoflavone

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Amentoflavone Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Amentoflavone Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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