1. Academic Validation
  2. Tyrosinase inhibitory components from the seeds of Cassia tora

Tyrosinase inhibitory components from the seeds of Cassia tora

  • Arch Pharm Res. 2018 May;41(5):490-496. doi: 10.1007/s12272-018-1032-4.
Ga Young Lee 1 Byoung Ok Cho 2 Jae Young Shin 2 Seon Il Jang 2 In Sook Cho 3 Hyo Young Kim 4 Ji Su Park 1 Chong Woon Cho 1 Jong Seong Kang 1 Jang Hoon Kim 5 Young Ho Kim 6
Affiliations

Affiliations

  • 1 College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.
  • 2 Department of Health Care & Science, Jeonju University, Jeonju, 55069, Republic of Korea.
  • 3 Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju, 55365, Republic of Korea.
  • 4 Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.
  • 5 Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea. oasis5325@gmail.com.
  • 6 College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea. yhk@cnu.ac.kr.
Abstract

Ten compounds (1-10) isolated from the seeds of Cassia tora were evaluated for Tyrosinase inhibition. Compounds 3, 4, and 7 inhibited Tyrosinase enzymatic activity in a dose-dependent manner, with IC50 values of 3.0 ± 0.8, 7.0 ± 0.4, and 9.2 ± 3.4 μM, respectively. Kinetic analyses revealed a mechanism consistent with competitive inhibition. In silico molecular docking showed that compounds 3 and 4 docked in the active site of Tyrosinase, whereas 7 interacted with Ala246 and Val248 at outside of the active site, and His244 and Glu256 at inside. Additionally, compounds 3, 4, and 7 suppressed melanogenesis in α-MSH-treated B16F10 melanoma cells at a concentration of 10 μM.

Keywords

Cassia tora; Competitive type; Melanogenesis; Molecular docking; Tyrosinase inhibitor.

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