2. Academic Validation
  3. Lambertellin from Pycnoporus sanguineus MUCL 51321 and its anti-inflammatory effect via modulation of MAPK and NF-κB signaling pathways

Lambertellin from Pycnoporus sanguineus MUCL 51321 and its anti-inflammatory effect via modulation of MAPK and NF-κB signaling pathways

  • Bioorg Chem. 2018 Oct:80:216-222. doi: 10.1016/j.bioorg.2018.06.021.
Jean-Bosco Jouda 1,  Emmanuel Mfotie Njoya 2,  Céline Djama Mbazoa 1,  ZongYuan Zhou 3,  Alain Meli Lannang 4,  Jean Wandji 1,  Yoshihito Shiono 5,  Fei Wang 6
Affiliations

Affiliations

  • 1 Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, P.O Box 812, Yaoundé, Cameroon.
  • 2 Key Laboratory of Natural Medicine and Clinical Translation, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China; Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O Box 812, Yaoundé, Cameroon.
  • 3 Key Laboratory of Natural Medicine and Clinical Translation, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
  • 4 Department of Chemistry, Higher Teachers' Training College, University Maroua, P. O Box 55, Maroua, Cameroon; Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Japan. Electronic address: alainmeli@yahoo.com.
  • 5 Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Japan.
  • 6 Key Laboratory of Natural Medicine and Clinical Translation, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China. Electronic address: wangfei@cib.ac.cn.
PMID: 29957490 DOI: 10.1016/j.bioorg.2018.06.021
Abstract

Lambertellin (1) and ergosta-5,7,22-trien-3-ol (2) were isolated from the solid rice fermentation of the plant pathogenic fungus Pycnoporus sanguineus MUCL 51321. Their structures were elucidated using comprehensive spectroscopic methods. The isolated compounds were tested on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Lambertellin (1) exhibited promising inhibitory activity against nitric oxide (NO) production with IC50 value of 3.19 µM, and it significantly inhibited the expression of inducible NO Synthase (iNOS) and cyclooxygenase 2 (COX-2). Lambertellin (1) also decreased the expression of pro-inflammatory cytokines IL-6 and IL-1β. The study of the mechanistic pathways revealed that lambertellin (1) exerts its anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophage cells by modulating the activation of the mitogen activated protein kinase (MAPK) and nuclear factor κB (NF-κB) signaling pathways. Therefore, lambertellin (1) could be a promising lead compound for the development of new anti-inflammatory drugs.

Keywords

Anti-inflammatory; Cytokines; Lambertellin; MAPKs; NF-κB; Nitric oxide; Pycnoporus sanguineus.

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