miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR

Background: The roles and related mechanism of miR-376a in breast Cancer cell progression are unclear.

Methods: Kaplan-Meier plotter analysis was used to analyze the correlation between miR-376a and the overall survival (OS) of breast Cancer patients. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect miR-376a level in breast Cancer cells. Cell viability, transwell migration and invasion, and cell Apoptosis were constructed to investigate the effects of miR-376a on breast Cancer cells. Luciferase reporter and RNA immunoprecipitation (RIP) were used to explore the targeting of miR-376a on NRP-1.

Results: miR-376a expression was positively correlated with the overall survival of breast Cancer patients, and significantly decreased in breast Cancer cells. Functionally, miR-376a over-expression suppressed cell proliferation, migration and invasion, and promoted cells Apoptosis. Additionally, miR-376a could directly target NRP-1 and exerted its effect through NRP-1.

Conclusion: miR-376a could suppress breast Cancer cell progression via directly targeting NRP-1.