2. Academic Validation
  3. Enhancement of Cell Adhesion, Cell Growth, Wound Healing, and Oxidative Protection by Gelatins Extracted from Extrusion-Pretreated Tilapia (Oreochromis sp.) Fish Scale

Enhancement of Cell Adhesion, Cell Growth, Wound Healing, and Oxidative Protection by Gelatins Extracted from Extrusion-Pretreated Tilapia (Oreochromis sp.) Fish Scale

  • Molecules. 2018 Sep 20;23(10):2406. doi: 10.3390/molecules23102406.
Chun-Yung Huang 1,  Tien-Chou Wu 2,  Yong-Han Hong 3,  Shu-Ling Hsieh 4,  Hui-Ru Guo 5,  Ren-Han Huang 6
Affiliations

Affiliations

  • 1 Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, Taiwan. cyhuang@nkust.edu.tw.
  • 2 Division of General Internal Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, No. 100, Tzyou 1st Rd., Sanmin District, Kaohsiung City 80708, Taiwan. 960552@ms.kmuh.org.tw.
  • 3 Department of Nutrition, I-Shou University (Yanchao Campus), No. 8, Yida Rd., Jiaosu Village, Yanchao District, Kaohsiung City 82445, Taiwan. yonghan@isu.edu.tw.
  • 4 Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, Taiwan. slhsieh@nkust.edu.tw.
  • 5 Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung City 81157, Taiwan. kuo19921122@gmail.com.
  • 6 Department of Nursing, Mackay Medical College, No. 46, Sec. 3, Zhongzheng Rd., Sanzhi District, New Taipei City 25245, Taiwan. lisa68850@gmail.com.
PMID: 30241285 DOI: 10.3390/molecules23102406
Abstract

Gelatin has been broadly utilized in the food, pharmaceutical, photographic, cosmetic and packaging industries, and there is also huge potential for novel applications of gelatin in the fields of biotechnology and biomedicine. In the present study, we extracted gelatin from fish processing waste, i.e., scale of tilapia, by a combined method of extrusion-pretreatment and hot water extraction. The extrusion-pretreatment process increases the extraction yield of gelatin. Three gelatins (FS2: preconditioning with double-distilled water (ddH₂O) before extrusion; FS12: preconditioning with citric acid solution before extrusion; FS14: preconditioning with acetic acid solution before extrusion) were obtained and all of them enhanced cell adhesion, cell growth, and wound healing in HaCaT cells and protected HaCaT cells from H₂O₂-induced cellular damage. Among FS2, FS12, and FS14, FS12 exhibited the most pronounced enhancement of cell adhesion, cell growth, and wound healing in HaCaT cells, and thus it may have potential as an effective natural raw material in cell therapies for cutaneous wounds and for reducing H₂O₂-induced oxidative damage of cells. In additional experiments, it was found that phosphorylations of Akt and mTOR are involved in the signaling pathway activated by FS2, FS12, and FS14 in HaCaT cells.

Keywords

Akt/mTOR pathway; cell adhesion; cell growth; extrusion; gelatin; human keratinocyte-derived cell line HaCaT; oxidative protection; tilapia; wound healing.

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