2. Academic Validation
  3. Hydrophobic drug adsorption loss to syringe filters from a perspective of drug delivery

Hydrophobic drug adsorption loss to syringe filters from a perspective of drug delivery

  • J Pharmacol Toxicol Methods. 2019 Jan-Feb;95:79-85. doi: 10.1016/j.vascn.2018.12.001.
Xiaoli Li 1,  Xiaoxu Ding 1,  Chengying Bian 1,  Shaoqun Wu 1,  Miao Chen 1,  Weizhuo Wang 1,  Jingjie Wang 1,  Lingyun Cheng 2
Affiliations

Affiliations

  • 1 Institute of Ocular Pharmacology, School of Ophthalmology and Optometry, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou, Zhejiang 325027, China.
  • 2 Institute of Ocular Pharmacology, School of Ophthalmology and Optometry, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou, Zhejiang 325027, China; Jacob's Retina Center at Shiley Eye Institute, Department of Ophthalmology, University of California San Diego, 9415 Campus Point Drive, La Jolla, CA 92037-0946, United States. Electronic address: l1cheng@ucsd.edu.
PMID: 30529169 DOI: 10.1016/j.vascn.2018.12.001
Abstract

Filtering with a syringe filter is a common operation in pharmaceutical analysis. Ophthalmic research often has a limited amount of sample and low amount of drug which is vulnerable to filtering membrane adsorption loss but not well recognized in the research community. Current study investigated drug adsorption by 11 types of syringe filters for 4 hydrophobic compounds commonly encountered in transscleral Drug Delivery. Among the 11 types of syringe filters surveyed, polytetrafluoroethylene - NBA, polytetrafluoroethylene - NLA, and polypropylene filters caused the least adsorptive drug loss for these four drugs studied. The magnitude of drug adsorption was filter- and drug-specific. Polytetrafluoroethylene-NLA caused the least adsorptive loss (1%) for triamcinolone acetonide, polytetrafluoroethylene-NBA caused the least adsorptive loss (5.4%) for diclofenac, and polypropylene caused the least adsorptive loss for cyclosporine A, 16.8% on average. Tacrolimus had the least adsorptive loss to the filters of polytetrafluoroethylene - NLA and polypropylene; however, the percentage of adsorptive loss from filtration was the highest (32% loss in average) among the four drugs surveyed. CONCLUSION: Low drug concentrations as seen in samples from ophthalmic researches are vulnerable to filtration drug loss. Selecting the right filter via validation is critical to avoid underestimating the drug level and distorting the resultant distribution/clearance profile in the ocular pharmacokinetic analysis.

Keywords

Adsorption drug loss; Hydrophobic drug; Syringe filter; Transscleral drug delivery.

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