2. Academic Validation
  3. Antiplasmodial and Cytotoxic Cytochalasins from an Endophytic Fungus, Nemania sp. UM10M, Isolated from a Diseased Torreya taxifolia Leaf

Antiplasmodial and Cytotoxic Cytochalasins from an Endophytic Fungus, Nemania sp. UM10M, Isolated from a Diseased Torreya taxifolia Leaf

  • Molecules. 2019 Feb 21;24(4):777. doi: 10.3390/molecules24040777.
Mallika Kumarihamy 1 2,  Daneel Ferreira 3,  Edward M Croom Jr 4,  Rajnish Sahu 5,  Babu L Tekwani 6,  Stephen O Duke 7,  Shabana Khan 8 9,  Natascha Techen 10,  N P Dhammika Nanayakkara 11
Affiliations

Affiliations

  • 1 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA. mkumarih@olemiss.edu.
  • 2 Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. mkumarih@olemiss.edu.
  • 3 Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. dferreir@olemiss.edu.
  • 4 Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. emcroom@olemiss.edu.
  • 5 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA. rsahu@alasu.edu.
  • 6 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA. btekwani@southenresearch.org.
  • 7 Natural Products Utilization Research Unit, USDA-ARS, University, MS 38677, USA. stephen.duke@ars.usda.gov.
  • 8 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA. skhan@olemiss.edu.
  • 9 Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. skhan@olemiss.edu.
  • 10 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA. ntechen@olemiss.edu.
  • 11 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA. dhammika@olemiss.edu.
PMID: 30795572 DOI: 10.3390/molecules24040777
Abstract

Bioassay-guided fractionation of an EtOAc extract of the broth of the endophytic fungus Nemania sp. UM10M (Xylariaceae) isolated from a diseased Torreya taxifolia leaf afforded three known cytochalasins, 19,20-epoxycytochalasins C (1) and D (2), and 18-deoxy-19,20-epoxy-cytochalasin C (3). All three compounds showed potent in vitro antiplasmodial activity and phytotoxicity with no cytotoxicity to Vero cells. These compounds exhibited moderate to weak cytotoxicity to some of the cell lines of a panel of solid tumor (SK-MEL, KB, BT-549, and SK-OV-3) and kidney epithelial cells (LLC-PK11). Evaluation of in vivo antimalarial activity of 19,20-epoxycytochalasin C (1) in a mouse model at 100 mg/kg dose showed that this compound had weak suppressive antiplasmodial activity and was toxic to Animals.

Keywords

Nemania; Torreya taxifolia; Xylariaceae; cytochalasins; cytotoxicity; malaria; phytotoxicity; plant pathogenic and endophytic fungi.

Figures
Products