2. Academic Validation
  3. Oxymatrine Inhibits Bocavirus MVC Replication, Reduces Viral Gene Expression and Decreases Apoptosis Induced by Viral Infection

Oxymatrine Inhibits Bocavirus MVC Replication, Reduces Viral Gene Expression and Decreases Apoptosis Induced by Viral Infection

  • Virol Sin. 2019 Feb;34(1):78-87. doi: 10.1007/s12250-019-00088-2.
Yanqin Ding 1,  Na Li 1,  Jinhan Sun 2,  Linran Zhang 1,  Jianhui Guo 1,  Xueqi Hao 3,  Yuning Sun 4
Affiliations

Affiliations

  • 1 Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China.
  • 2 School of Clinical Medicine, Hainan Medical University, Haikou, 571199, China.
  • 3 Department of Respiratory and Critical Care, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan, 750004, China.
  • 4 Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China. sunraining2008@hotmail.com.
PMID: 30805776 DOI: 10.1007/s12250-019-00088-2
Abstract

Oxymatrine (OMT), as the main active component of Sophoraflavescens, exhibits a variety of pharmacological properties, including anti-oxidative, anti-inflammatory, anti-tumor, and anti-viral activities, and currently is extensively employed to treat viral hepatitis; however, its effects on parvovirus Infection have yet to be reported. In the present study, we investigated the effects of OMT on cell viability, virus DNA replication, viral gene expression, cell cycle, and Apoptosis in Walter Reed canine cells/3873D infected with minute virus of canines (MVC). OMT, at concentrations below 4 mmol/L(no cellular toxicity), was found to inhibit MVC DNA replication and reduce viral gene expression at both mRNA and protein levels, which was associated with the inhibition of cell cycle S-phase arrest in early-stage of MVC Infection. Furthermore, OMT significantly increased cell viability, decreased MVC-infected cell Apoptosis, and reduced the expression of activated Caspase 3. Our results suggest that OMT has potential application in combating parvovirus Infection.

Keywords

Apoptosis; Infection; Minute virus of canines (MVC); Oxymatrine (OMT); Replication.

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