2. Academic Validation
  3. Liquefaction of chicken sternal cartilage by steam explosion to isolate chondroitin sulfate

Liquefaction of chicken sternal cartilage by steam explosion to isolate chondroitin sulfate

  • Carbohydr Polym. 2019 Jul 1;215:73-81. doi: 10.1016/j.carbpol.2019.03.032.
Qingshan Shen 1,  Chunhui Zhang 2,  Wei Jia 3,  Xiaojie Qin 3,  Xiong Xu 3,  Mengliang Ye 3,  Haizhen Mo 4,  Aurore Richel 5
Affiliations

Affiliations

  • 1 Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.
  • 2 Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: dr_zch@163.com.
  • 3 Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
  • 4 Department of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.
  • 5 University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.
PMID: 30981372 DOI: 10.1016/j.carbpol.2019.03.032
Abstract

Chondroitin sulfate (CS), together with peptide, was isolated from the liquid fraction of chicken sternal cartilage subjected to steam explosion (SE) by membrane separation. Cartilage was liquefied via the SE conditions, including various pressures (1.0-1.6 MPa) and times (60-140 s). The extraction procedure was optimized as follows: the amount of papain added, 0.11%; enzymolysis time, 10.5 h; and enzymolysis temperature, 56.5 °C, under which the highest recovery and total yield of CS were 92.15% and 18.55% at 1.4 MPa for120 s, and the counterparts of Peptides were 87.35% (1.0 MPa, 140 s) and 63.07% (1.6 MPa, 140 s). The average molecular weight of CS samples ranged from 30 to 35 kDa. CS sample was confirmed using agarose-gel electrophoresis, and the structure was analysed Fourier transform infrared spectroscopy, chromatography and nuclear magnetic resonance. Taken together, SE can be an eco-friendly pretreatment method to liquefy cartilage for CS isolation.

Keywords

Cartilage; Chondroitin sulfate; Liquefaction; Steam explosion.

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