2. Academic Validation
  3. Cognitive-enhancing and ameliorative effects of acanthoside B in a scopolamine-induced amnesic mouse model through regulation of oxidative/inflammatory/cholinergic systems and activation of the TrkB/CREB/BDNF pathway

Cognitive-enhancing and ameliorative effects of acanthoside B in a scopolamine-induced amnesic mouse model through regulation of oxidative/inflammatory/cholinergic systems and activation of the TrkB/CREB/BDNF pathway

  • Food Chem Toxicol. 2019 Jul;129:444-457. doi: 10.1016/j.fct.2019.04.062.
Govindarajan Karthivashan 1,  Mee-Hyang Kweon 2,  Shin-Young Park 3,  Joon-Soo Kim 4,  Deuk-Hoi Kim 5,  Palanivel Ganesan 6,  Dong-Kug Choi 7
Affiliations

Affiliations

  • 1 Department of Applied Life Sciences, Graduate School of Konkuk University, Research Institute of Inflammatory Diseases, Chungju, 27478, Republic of Korea. Electronic address: karthivashan@gmail.com.
  • 2 Research Center, Phyto Corporation, Seoul, 08826, Republic of Korea. Electronic address: mhkweon@korea.ac.kr.
  • 3 Department of Applied Life Sciences, Graduate School of Konkuk University, Research Institute of Inflammatory Diseases, Chungju, 27478, Republic of Korea. Electronic address: ifresha@nate.com.
  • 4 Department of Applied Life Sciences, Graduate School of Konkuk University, Research Institute of Inflammatory Diseases, Chungju, 27478, Republic of Korea. Electronic address: kgfdkr@gmail.com.
  • 5 Research Center, Phyto Corporation, Seoul, 08826, Republic of Korea. Electronic address: dhkim@phytoco.com.
  • 6 Department of Integrated Bio Science and Biotechnology, College of Biomedical and Health Science, Nanotechnology Research Center, Konkuk University, Chungju, 27478, Republic of Korea. Electronic address: palanivel67@gmail.com.
  • 7 Department of Applied Life Sciences, Graduate School of Konkuk University, Research Institute of Inflammatory Diseases, Chungju, 27478, Republic of Korea; Department of Integrated Bio Science and Biotechnology, College of Biomedical and Health Science, Nanotechnology Research Center, Konkuk University, Chungju, 27478, Republic of Korea. Electronic address: choidk@kku.ac.kr.
PMID: 31077737 DOI: 10.1016/j.fct.2019.04.062
Abstract

Recently, our research team reported the anti-amnesic potential of desalted-hydroethanolic extracts of Salicornia europaea L. (SE-EE). In this study, we performed bioactivity-guided isolation and identification of Acanthoside B (Aca.B), from SE-EE, as the potential bioactive candidate and examined anti-amnesic activity with its potential mechanism of action using an in vivo model. S7-L3-3 purified from SE-EE showed enhanced in vitro acetylcholinesterase (AChE) inhibitory activity. The isolated S7-L3-3 was identified and characterized as Aca.B using varied spectral analyses, i.e., Nuclear magnetic resonance (NMR), Ultraviolet-visible (UV-Vis), and Electrospray ionization-mass spectrometry (ESI-MS). In the in vitro studies, Aca.B exhibited negligible toxicity and showed a dose-dependent nitric oxide inhibitory potential in Lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. In the in vivo studies, the oral administration of Aca.B to mice showed enhanced bioavailability and dose-dependent repression of the behavioral/cognitive impairment by regulating the cholinergic function, restoring the antioxidant status, attenuating the inflammatory cytokines/mediators and actively enriching neurotropic proteins in the hippocampal regions of the scopolamine-administered mice.

Keywords

Amnesia; Anti-AChE; Anti-inflammation; Antioxidant; Salicornia europaea L; Scopolamine; TrkB/CREB/BDNF.

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