Acanthoside B

Name: Acanthoside B
Brand: MedChemExpress
SKU: HY-N2807
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2807 Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Acanthoside B is a potential bioactive lignan with anti-inflammatory and anti-amnesic activities. Acanthoside B can be used for alzheimer's disease and lung inflammation research

For research use only. We do not sell to patients.

Acanthoside B Chemical Structure

CAS No. : 7374-79-0

Size	Price	Stock	Quantity
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 490	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Acanthoside B is a potential bioactive lignan with anti-inflammatory and anti-amnesic activities. Acanthoside B can be used for alzheimer's disease and lung inflammation research^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
BMDC	IC₅₀	> 50 μM Compound: 10	Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 10	Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 10	Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BV-2	IC₅₀	3.08 μM Compound: 17	Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction	[PMID: 27623545]
Caco-2	IC₅₀	< 5 μg/mL Compound: page no 2475, R9C1	Cytotoxicity against human Caco2 cells after 72 hrs by MTS assay Cytotoxicity against human Caco2 cells after 72 hrs by MTS assay	[PMID: 22032697]
CCD-18Co	IC₅₀	< 5 μg/mL Compound: page no 2475, R9C1	Cytotoxicity against human CCD-18Co cells after 72 hrs by MTS assay Cytotoxicity against human CCD-18Co cells after 72 hrs by MTS assay	[PMID: 22032697]
HCT-116	IC₅₀	< 5 μg/mL Compound: page no 2475, R9C1	Cytotoxicity against human HCT116 cells after 72 hrs by MTS assay Cytotoxicity against human HCT116 cells after 72 hrs by MTS assay	[PMID: 22032697]

In Vivo

Acanthoside B (oral gavage; 10-20 mg/kg; 7 days prior to Scopolamine injection) attenuates Scopolamine inflicted AD-like amnesic traits by restoring the cholinergic activity, decreasing the endogenous antioxidant status, suppressing neuroinflammation, and activating the TrkB/CREB/BDNF pathway in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Scopolamine-induced amnesic mouse model^[1]
Dosage:	10 mg/kg; 20 mg/kg
Administration:	oral gavage; 7 days
Result:	Exhibited an anti-amnesic effect in mice.

Molecular Weight

580.58

Formula

C₂₈H₃₆O₁₃

CAS No.

7374-79-0

Appearance

Solid

Color

White to off-white

SMILES

COC1=C(O[C@@H]2O[C@@H]([C@H]([C@@H]([C@H]2O)O)O)CO)C(OC)=CC([C@]3([H])[C@]4([H])[C@@](CO3)([H])[C@](C5=CC(OC)=C(C(OC)=C5)O)([H])OC4)=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (172.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7224 mL	8.6121 mL	17.2242 mL
5 mM	0.3445 mL	1.7224 mL	3.4448 mL
10 mM	0.1722 mL	0.8612 mL	1.7224 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (280 KB) SDS (251 KB)

COA (261 KB) HNMR (228 KB) LCMS (257 KB)

Handling Instructions (2659 KB)

References

[1]. Govindarajan Karthivashan, et al. Cognitive-enhancing and ameliorative effects of acanthoside B in a scopolamine-induced amnesic mouse model through regulation of oxidative/inflammatory/cholinergic systems and activation of the TrkB/CREB/BDNF pathway. Foo [Content Brief]

[2]. Ju Hee Lee, et al. Inhibition of Lung Inflammation by Acanthopanax divaricatus var. Albeofructus and Its Constituents. Biomol Ther (Seoul). 2016 Jan;24(1):67-74. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7224 mL	8.6121 mL	17.2242 mL	43.0604 mL
	5 mM	0.3445 mL	1.7224 mL	3.4448 mL	8.6121 mL
	10 mM	0.1722 mL	0.8612 mL	1.7224 mL	4.3060 mL
	15 mM	0.1148 mL	0.5741 mL	1.1483 mL	2.8707 mL
	20 mM	0.0861 mL	0.4306 mL	0.8612 mL	2.1530 mL
	25 mM	0.0689 mL	0.3445 mL	0.6890 mL	1.7224 mL
	30 mM	0.0574 mL	0.2871 mL	0.5741 mL	1.4353 mL
	40 mM	0.0431 mL	0.2153 mL	0.4306 mL	1.0765 mL
	50 mM	0.0344 mL	0.1722 mL	0.3445 mL	0.8612 mL
	60 mM	0.0287 mL	0.1435 mL	0.2871 mL	0.7177 mL
	80 mM	0.0215 mL	0.1077 mL	0.2153 mL	0.5383 mL
	100 mM	0.0172 mL	0.0861 mL	0.1722 mL	0.4306 mL

Acanthoside B Related Classifications

Neurological Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Acanthoside B7374-79-0OthersAlzheimer's diseaseADAnti-Inflammatorychronic obstructive pulmonary diseaseCOPDInhibitorinhibitorinhibit

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Acanthoside B

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Complete Stock Solution Preparation Table

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